Objectives: To develop a "miniorgan" culture of human endometrium dated according to the LH peak in order to study the process of implantation of a human blastocyst in vitro.
Design: Preliminary results of a more extensive study with the same objectives.
Setting: Hospital-based unit of reproductive health and university-related reproductive research laboratories.
Patients: Six apparently healthy women with normal regular menstrual cycles providing endometrial material 4, 5 and 6 days after the LH peak.
Intervention: One part of the biopsy was used for histologic dating. The other part was incubated in culture medium RPMI-1640 and exposed to the contact with one to three embryos fertilized in vitro.
Main Outcome Measure: Biopsy material before and after incubation was studied by morphometric methods in a light microscope. The histologic changes were recorded by photomicrographs.
Results: Implantation of an embryo obtained 4 days after IVF penetrated the lining endometrial epithelium of a biopsy obtained 4 days after the LH peak and kept in vitro for 24 hours. The embryo was placed on the lining epithelium of the endometrium within 3 hours after the biopsy was taken.
Conclusion: A new method to study the process of implantation of a human embryo in vitro has been described, allowing the embryo to penetrate the lining endometrial epithelium in a biopsy obtained at LH +4/+5.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0015-0282(16)58291-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gene expression and mucilage adaptations to salinity in germination of extreme halophyte Schrenkiella parvula seeds.
Plant Physiol Biochem
January 2025
Department of Biology, Faculty of Science, Ege University, Izmir, Türkiye. Electronic address:
Salinization is a significant global issue causes irreversible damage to plants by reducing osmotic potential, inhibiting seed germination, and impeding water uptake. Seed germination, a crucial step towards the seedling stage is regulated by several hormones and genes, with the balance between abscisic acid and gibberellin being the key mechanism that either promotes or inhibits this process. Additionally, mucilage, a gelatinous substance, is known to provide protection against drought, herbivory, soil adhesion, and seed sinking.
View Article and Find Full Text PDFSoybean tolerance to waterlogging is achieved by detoxifying root lactate via lactate dehydrogenase in leaves and metabolizing malate and succinate.
Plant Physiol Biochem
January 2025
Botany Department, Federal University of Pelotas, Capão Do Leão, RS, 96160-000, Brazil.
Waterlogging is a significant stressor for crops, particularly in lowland regions where soil conditions exacerbate the problem. Waterlogged roots experience hypoxia, disrupting oxidative phosphorylation and triggering metabolic reorganization to sustain energy production. Here, we investigated the metabolic aspects that differentiate two soybean sister lines contrasting for waterlogging tolerance.
View Article and Find Full Text PDFHybrid Data Augmentation Strategies for Robust Deep Learning Classification of Corneal Topographic MapTopographic Map.
Biomed Phys Eng Express
January 2025
National School of Electronics and Telecommunication of Sfax, Sfax rte mahdia, sfax, sfax, 3012, TUNISIA.
Deep learning has emerged as a powerful tool in medical imaging, particularly for corneal topographic map classification. However, the scarcity of labeled data poses a significant challenge to achieving robust performance. This study investigates the impact of various data augmentation strategies on enhancing the performance of a customized convolutional neural network model for corneal topographic map classification.
View Article and Find Full Text PDFExploring the Needs and Preferences of Users and Parents to Design a Mobile App to Deliver Mental Health Peer Support to Adolescents With Type 1 Diabetes: Qualitative Study.
JMIR Diabetes
January 2025
Research Institute, BC Children's Hospital, Vancouver, BC, Canada.
Background: Beyond physical health, managing type 1 diabetes (T1D) also encompasses a psychological component, including diabetes distress, that is, the worries, fears, and frustrations associated with meeting self-care demands over the lifetime. While digital health solutions have been increasingly used to address emotional health in diabetes, these technologies may not uniformly meet the unique concerns and technological savvy across all age groups.
Objective: This study aimed to explore the mental health needs of adolescents with T1D, determine their preferred modalities for app-based mental health support, and identify desirable design features for peer-delivered mental health support modeled on an app designed for adults with T1D.
Psychological and Behavioral Insights From Social Media Users: Natural Language Processing-Based Quantitative Study on Mental Well-Being.
JMIR Form Res
January 2025
Smith School of Business, Queen's University, Kingston, ON, Canada.
Background: Depression significantly impacts an individual's thoughts, emotions, behaviors, and moods; this prevalent mental health condition affects millions globally. Traditional approaches to detecting and treating depression rely on questionnaires and personal interviews, which can be time consuming and potentially inefficient. As social media has permanently shifted the pattern of our daily communications, social media postings can offer new perspectives in understanding mental illness in individuals because they provide an unbiased exploration of their language use and behavioral patterns.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!