The objective of this study was to apply the 32P-postlabelling method for the detection of UV-induced dipyrimidine photoadducts in human skin explants. [32P]TLC and [32P]HPLC analysis were used. These techniques allow detection of different combinations of cyclobutane dimers and 6-4 photoproducts in human skin at low doses of irradiation. Detection limit of the present method for some dinucleotide adducts is < 1 adduct per 10(7) normal nucleotides. Reaction of photochemical reversion of the cyclobutane dimers was performed to confirm the nature of the products detected in human skin explants.
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| http://dx.doi.org/10.1093/carcin/16.12.3015 | DOI Listing |
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