This paper reports on the development of a motility measuring system for human subgingival microflora using a high speed shutter camera and an image analysis system. The paper first presents the method for estimating the motility of subgingival microflora by the total number of pixels and then describes the evaluation of the total number of pixels as an indicator of bacterial motility. Our results with this system demonstrate that the motility of subgingival microflora can be expressed as the total number of pixels.

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