The paper presents the radiorespirometric and enzymatic findings of the insensitivity of catabolism of glucose and acetate in Y. pseudotuberculosis cells. The reduction in a Y. pseudotuberculosis cultivation temperature (4 degrees C) has been found to be followed by higher activities of NADP(+)-dependent dehydrogenases and increased functioning of the glyoxylate cycle. The temperature-compensated increase in the functioning intensity of alternative metabolic pathways appears to be part of adaptative metabolic manifestations in the Y. pseudotuberculosis cells during autonomic habitance and accumulation in the environmental objects.
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PLoS One
December 2024
Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, College of Life Sciences, Jilin Agricultural University, Changchun, China.
Outer membrane vesicles (OMVs) are immunogenic self-adjuvanting vesicles produced by Gram-negative bacteria such as Pseudomonas aeruginosa and Yersinia pseudotuberculosis. While the effects of OMVs on different antigens immune stimulation are not clear. In this study, we constructed recombinant Yersinia pseudotuberculosis ΔlpxL strain,with pBlue-PcrV and pBlue-OprF/I, and then purified ΔlpxL rOMVPcrV (rOMVyp2P)and ΔlpxL rOMVOprF/I (rOMVyp2F) and analyzed its effect on immune response and protection against Pseudomonas aeruginosa PAO1 infection.
View Article and Find Full Text PDFOpen Biol
November 2024
School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, UK.
Gram-negative pathogens pose a significant threat due to their propensity for causing various infections, often coupled with formidable resistance to conventional antibiotic treatments. The development of antivirulence (AV) compounds emerges as a promising alternative strategy by disrupting virulence mechanisms rather than targeting bacterial viability. Aurodox has exhibited promising AV properties in previous studies by blocking the expression and function of the LEE-encoded type 3 secretion system (T3SS) in enterohaemorrhagic , an injectosome that translocates effector proteins directly into host target cells.
View Article and Find Full Text PDFJ Immunol
December 2024
Department of Pathology, Immunology and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Rutgers-The State University of New Jersey, Newark, NJ.
IL-33 plays an important role in the early programming of CD8 T cells; however, its contribution to the differentiation of tissue-resident memory T cells in vivo remains poorly defined. After infection of mice with Yersinia pseudotuberculosis, IL-33 expression was increased in the intestinal tissue, and this coincided with the expression of ST2 on T cells infiltrating the intestinal epithelium and lamina propria. Blocking IL-33 signaling after T cell infiltration of the intestinal tissue did not significantly impact the number or phenotype of tissue-resident memory T cells generated.
View Article and Find Full Text PDFbioRxiv
September 2024
W. Harry Feinstone Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
It has been long appreciated that expression of the type-III secretion system (T3SS) in culture is associated with growth arrest. Here we sought to understand whether this impacts expression of ribosomal protein genes, which were among the most highly abundant transcripts in exponential phase based on RNA-seq analysis. To visualize changes in ribosomal protein expression, we generated a fluorescent transcriptional reporter with the promoter upstream of /S10 fused to a destabilized variant.
View Article and Find Full Text PDFVaccine
October 2024
Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil. Electronic address:
Sheep farming contributes to the socioeconomic development of small and medium-scale livestock farmers. However, several factors can hinder successful animal production, as is the case for infectious diseases, such as the one caused by Corynebacterium pseudotuberculosis, known as caseous lymphadenitis (CLA). CLA has >90% prevalence in Brazilian herds and antibiotic treatment is not effective, consequently causing significant economic losses to farmers.
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