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Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris.
Sci Rep
January 2025
Department of Biotechnology, Jožef Stefan Institute, Ljubljana, Slovenia.
Extracellular vesicles (EVs) are nanosized lipid bilayer particles released by various cellular organisms that carry an array of bioactive molecules. EVs have diagnostic potential, as they play a role in intercellular interspecies communication, and could be applied in drug delivery. In contrast to mammalian cell-derived EVs, the study of EVs from bacteria, particularly Gram-positive bacteria, received less research attention.
View Article and Find Full Text PDFExpanding the genetic toolbox for the obligate human pathogen .
Front Bioeng Biotechnol
June 2024
Max Planck Unit for the Science of Pathogens, Berlin, Germany.
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen To overcome this limitation and enable the straightforward investigation of gene functions in , we have developed a comprehensive genetic toolset. By adapting and combining different tools previously applied in other Gram-positive bacteria, we have created new replicative and integrative plasmids for gene expression and genetic manipulation, constitutive and inducible promoters as well as fluorescence reporters for .
View Article and Find Full Text PDFDiscovering genetic determinants for cell-to-cell adhesion in two prevalent conjugative lactococcal plasmids.
Curr Res Microb Sci
April 2024
School of Microbiology & APC Microbiome Ireland, University College Cork, Western Road, Cork, Ireland.
Plasmids pNP40 and pUC11B encode two prevalent yet divergent conjugation systems, which have been characterized in detail recently. Here, we report the elucidation of the putative adhesins of the pNP40 and pUC11B conjugation systems, encoded by and , respectively. Despite their significant sequence divergence, TraAd and TrsAd represent the most conserved component between the pNP40- and the pUC11B-encoded conjugation systems and share similar peptidoglycan-hydrolase domains.
View Article and Find Full Text PDFBacterial proteome adaptation during fermentation in dairy environments.
Food Microbiol
August 2024
Host-Microbe Interactomics, Wageningen University & Research, Wageningen, the Netherlands; TI Food and Nutrition, Wageningen, the Netherlands. Electronic address:
The enzymatic repertoire of starter cultures belonging to the Lactococcus genus determines various important characteristics of fermented dairy products but might change in response to the substantial environmental changes in the manufacturing process. Assessing bacterial proteome adaptation in dairy and other food environments is challenging due to the high matrix-protein concentration and is even further complicated in particularly cheese by the high fat concentrations, the semi-solid state of that matrix, and the non-growing state of the bacteria. Here, we present bacterial harvesting and processing procedures that enable reproducible, high-resolution proteome determination in lactococcal cultures harvested from laboratory media, milk, and miniature Gouda cheese.
View Article and Find Full Text PDFRecombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus).
Fish Shellfish Immunol
June 2024
Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia. Electronic address:
Article Synopsis
- Streptococcus agalactiae infections in tilapia aquaculture lead to high mortality and significant economic losses, prompting farmers to use antibiotics, which fosters drug resistance.
- A new live recombinant L. lactis vaccine expressing immunogenic proteins was developed and tested orally on juvenile tilapia, demonstrating that it can generate immune responses and significantly reduce mortality rates.
- The tSIP version of the vaccine showed the best protection, with a relative percentage of survival of 89% against S. agalactiae, suggesting its potential as an effective oral vaccine for tilapia aquaculture.
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