Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0168-9525(00)89085-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evidence That Aquaporin 11 (AQP11) in the Spiny Dogfish () May Represent a Pseudogene.
Int J Mol Sci
February 2024
Biology Department, Georgia Southern University, P.O. Box 8042, Statesboro, GA 30460, USA.
Various attempts to amplify an AQP11 cDNA from tissues of the spiny dogfish () were made. Two pairs of deoxy-inosine-containing degenerate primers were designed based on conserved amino acid sequences from an AQP11 alignment. These primers yielded some faint bands from gill cDNA that were sequenced.
View Article and Find Full Text PDFAssessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences.
Clin Epigenetics
December 2017
Department of Pathology, The University of Melbourne, Parkville, Victoria 3010 Australia.
Background: Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status of any given epiallele. This is highly dependent on primer design.
View Article and Find Full Text PDFMolecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR.
Methods Mol Biol
December 2016
Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunizationand Respiratory Disease, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Virologic surveillance is essential to the success of the World Health Organization initiative to eradicate poliomyelitis. Molecular methods have been used to detect polioviruses in tissue culture isolates derived from stool samples obtained through surveillance for acute flaccid paralysis. This chapter describes the use of realtime PCR assays to identify and serotype polioviruses.
View Article and Find Full Text PDFInosine at Different Primer Positions to Study Structure and Diversity of Prokaryotic Populations.
Curr Issues Mol Biol
June 2016
Department of Life Sciences, Achva Academic College, MP Shikmim, 79800, Israel.
Culture-independent methods, employed to study the diversity and complexity of microbial communities that are based on amplification of rRNA genes with universal primers, include gradient gel electrophoresis (denaturing or temperature), single-strand-conformation polymorphism, restriction fragment length polymorphism, qPCR and high-throughput DNA sequencing. Substituting one or more base(s) within or at the 3'-termi of the universal primers by inosine can overcome some of their shortcomings improving amplification capacity. Universal primer sets do not usually amplify sequences with nucleotide mismatch to the templates, particularly in the last three bases, whereas inosine-modified primers anneal and amplify a variety of rRNA gene sequences.
View Article and Find Full Text PDFDetection of Banana mild mosaic virus and Banana virus X by polyvalent degenerate oligonucleotide RT-PCR (PDO-RT-PCR).
J Virol Methods
June 2007
CIRAD, UPR75, Station de Neufchâteau, F-97130 Capesterre Belle-Eau, FWI, Guadeloupe.
Viruses are important constraints to the movement and propagation of plant germplasm, especially for vegetatively propagated crops such as banana and plantain. Their control relies primarily on the use of virus-free plant material, whose production and certification requires sensitive and reliable detection methods. An existing polyvalent degenerate oligonucleotide RT-PCR (PDO-RT-PCR) assay was adapted to the detection of Banana mild mosaic virus (BanMMV) and Banana virus X, two Flexiviridae infecting Musa spp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!