Chemical heterogeneity in adult rat cerebellar Purkinje cells as revealed by zebrin I and low-affinity nerve growth factor receptor immunocytochemical expression following injury.

Cerebellar Purkinje cells in rat express low-affinity nerve growth factor receptor during development, but rarely in normal adult animals. However, after either mechanical injury or colchicine treatment during adulthood, these cells re-express low-affinity nerve growth factor receptor-immunoreactive protein. Two Purkinje cell subpopulations were defined in normal adult cerebellum by the presence or the absence of zebrin I antigen. Nevertheless, it remains an open question as to whether low-affinity nerve growth factor receptor-immunoreactive protein can be expressed by all damaged Purkinje cells, independent of their location and their staining with antibodies against intrinsic molecular markers that reveal Purkinje cell heterogeneity, such as zebrin I. In this study, a serial-section immunocytochemical mapping of the expression zebrin I and low-affinity nerve growth factor receptor, using specific monoclonal antibodies, we carried out in colchicine-treated rats. After mechanical damage of the cerebellar cortex, co-localization of these antigens at the cellular level was also analysed in thin adjacent sections, and by using a combined immunocytochemical staining method in individual sections. The findings revealed the existence of three sub-sets of Purkinje cells: (1) two complementary groups distinctly immunoreactive to one antibody, but not to the other and (2) a third group that contained double-labelled cells. In contrast, co-expression of both antigens was never observed following mechanical lesions. The seemingly independent response to mechanical injury of Purkinje cells located in different zebrin-defined compartments, indicates that particular subpopulations of Purkinje cells may respond differentially to traumatic injury.