Five peptides corresponding to human lutropin (hLH) subunit fragments were synthesized by a solid phase method and their physicochemical characteristics are presented. Antibodies induced in rabbits with 3 peptides of beta hLH fragments coupled to thyrotropin did not bind hLH and human chorionic gonadotropin (hCG). Also 2 synthetic peptides of alpha hLH fragments did not react with rabbit anti-hLH, anti-hCG and anti-alpha hCG antibodies. Using 2 monoclonal anti-alpha hCG and anti-beta hLH antibodies a sensitive sandwich ELISA technique was elaborated. Using this technique stability of hLH was investigated. The ELISA was also applied for assays of urine hLH in normal and ovulation days.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effect of Curcumin on Lipid Mediators, Glycemic Index, and Oxidative Stress and Inflammation Biomarkers in Polycystic Ovary Syndrome: Future Directions and Current Knowledge - A Systematic Revie.
Prostaglandins Other Lipid Mediat
January 2025
Department of Biology, College Of Education For Pure Sciences, University Of Anbar, Ramadi 31001, Anbar, Iraq.
Polycystic ovary syndrome (PCOS) is one of the most common and important polygenic endocrine disorders among women of reproductive-aged. Current treatments are mostly used only to control the signs and symptoms of the disease, while not being able to completely prevent complications. Curcumin is one of the active compounds in turmeric, which is commonly used for a wide range of metabolic and inflammatory diseases.
View Article and Find Full Text PDFInfluence of Menstrual Phase and Symptoms on Sleep Before and After Matches for Professional Footballers.
Scand J Med Sci Sports
January 2025
School of Sport, Exercise and Rehabilitation, Faculty of Health, University of Technology Sydney, Ultimo, Australia.
This study investigated the association of menstrual cycle phase and symptoms with objective and subjective sleep measures from professional footballers before and after matches. Twenty-three non-hormonal contraceptive-using professional footballers (from four clubs) were monitored for up to four menstrual cycles during a domestic league season. Menstrual phases (menstruation, mid-late follicular, luteal) were determined using calendar counting and urinary hormone tests (luteinizing hormone and pregnandiol-3-glucuronide).
View Article and Find Full Text PDFThe role of luteinizing hormone activity in spermatogenesis: from physiology to clinical practice.
Reprod Biol Endocrinol
January 2025
Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark.
The production of spermatozoa, a process known as spermatogenesis, is primarily controlled by follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-driven testosterone. LH acts on the Leydig cells, stimulating steroid production, predominantly testosterone, and activating critical inter-related spermatogenesis regulatory pathways. Despite evidence that exogenous gonadotropins containing LH activity can effectively restore spermatogenesis in males with hypogonadotropic hypogonadism, the use of these drugs to treat other forms of male infertility is the subject of an ongoing debate.
View Article and Find Full Text PDFThe kinetically-derived maximal dose (KMD) is defined as the maximum external dose at which kinetics are unchanged relative to lower doses, e.g., doses at which kinetic processes are not saturated.
View Article and Find Full Text PDFEndocrine Hormones and Their Impact on Pubertal Gynecomastia.
J Clin Med
December 2024
Department of Aesthetic and Reconstructive Breast Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100144, China.
Pubertal gynecomastia (PG) is a common condition characterized by the abnormal development and hyperplasia of unilateral or bilateral breast tissue in adolescent males, affecting up to 50% of appropriately aged adolescents and exhibiting rising prevalence over recent years. The etiology of PG is multifaceted, encompassing physiological, pharmacological, and pathological factors. This narrative review synthesizes evidence from a comprehensive selection of peer-reviewed literature, including observational studies, clinical trials, systematic reviews, and case reports, to explore the pivotal role of endocrine hormones in the pathogenesis of PG.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!