Dermatophytes are usually isolated from house dust by using actidione-chloramphenicol Sabouraud glucose medium (ACS medium). We have prepared a new medium, 5FC medium, by adding gentamicin sulphate (GS) and 5-fluorocytosine (5FC) to ACS medium to achieve more efficient isolation of dermatophytes from house dust. 5FC medium was more effective in isolating dermatophytes than ACS medium or ACS medium supplemented with GS alone. Trichophyton rubrum could be grown from 13 out of 19 house dust samples from the homes of patients with tinea pedis (68.4%), and T. mentagrophytes could be grown from 17 out of 21 (81.0%). Two of 20 house dust samples from the home of a control family without dermatophytosis grew only one colony of dermatophytes in 5FC medium; the rest of the samples showed no growth. The number of colonies isolated on 5FC medium was much higher than on ACS medium (5.3 vs. 2.0 for T. rubrum and 17.2 vs. 2.1 for T. mentagrophytes). In addition, the size of the isolated colonies was much larger than that on ACS medium. Thus, 5FC medium can be regarded as a useful tool for isolating dermatophytes from various contaminated samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1439-0507.1995.tb00072.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Autologous Peripheral Blood-Derived Orthobiologics for the Management of Elbow Disorders: A Review of Current Clinical Evidence.
Pain Ther
January 2025
Department of Trauma and Orthopaedic Surgery, Faculty of Medicine and Psychology, University La Sapienza, 00185, Rome, Italy.
Introduction: Elbow ailments are common, but conventional treatment modalities have shortcomings, offering only interim pain relief rather than targeting the underlying pathophysiology. The last two decades have seen a marked increase in the use of autologous peripheral blood-derived orthobiologics (APBOs), such as platelet-rich plasma (PRP), to manage elbow disorders. Platelet-rich plasma (PRP) is the most widely used APBO, but its efficacy remains debatable.
View Article and Find Full Text PDFSubventricular Accumulation of Cu in the Aging Mouse Brain Does Not Associate with Anticipated Increases in Markers of Oxidative Stress.
ACS Chem Neurosci
January 2025
School of Molecular and Life Sciences, Faculty of Science and Engineering, Curtin University, Bentley, WA 6845, Australia.
Natural aging is associated with mild memory loss and cognitive decline, and age is the greatest risk factor for neurodegenerative diseases, such as Alzheimer's disease. There is substantial evidence that oxidative stress is a major contributor to both natural aging and neurodegenerative disease, and coincidently, levels of redox active metals such as Fe and Cu are known to be elevated later in life. Recently, a pronounced age-related increase in Cu content has been reported to occur in mice and rats around a vital regulatory brain region, the subventricular zone of lateral ventricles.
View Article and Find Full Text PDFDesign of RuO Electrocatalysts Containing Metallic Ru on the Surface to Accelerate the Alkaline Hydrogen Evolution Reaction.
ACS Appl Mater Interfaces
January 2025
Key Laboratory for Advanced Ceramics and Machining Technology of Ministry of Education, Institute of New-Energy, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
The development of water splitting technology in alkaline medium requires the exploration of electrocatalysts superior to Pt/C to boost the alkaline hydrogen evolution reaction (HER). Ruthenium oxides with strong water dissociation ability are promising candidates; however, the lack of hydrogen combination sites immensely limits their performance. Herein, we reported a unique RuO catalyst with metallic Ru on its surface through a simple cation exchange method.
View Article and Find Full Text PDFBiomimetic Air-Lifted Organ Culture System with a Protective Coverage Membrane for Full-Thickness Corneal Preservation.
ACS Biomater Sci Eng
January 2025
Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
Effective storage and utilization of limited donor corneal resources are in high demand to alleviate the shortage of donor corneal tissue. Here, we designed a static air-lifted organ culture system equipped with a protective coverage membrane, namely, an air-lifted OC-P system, to provide a biomimetic physiological environment for full-thickness corneal preservation. The air-lifted OC-P system features a unique collagen-based protective coverage membrane that can offer a moist, oxygen-rich environment for corneal epithelium, produce an appropriate intraocular pressure onto the cornea by gravity, and facilitate the maintenance of the organ culture medium level for nutrient supply during corneal preservation.
View Article and Find Full Text PDFZinc Doped Akermanite: A Promising Biomaterial for Orthopedic Application with Enhanced Bioactivity, Mechanical Strength, and Bacterial Study.
ACS Omega
January 2025
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, India.
Incorporating zinc into biocompatible materials has been identified as a potential strategy for promoting bone regeneration and osteogenic activity during hard tissue regeneration. This work aimed to investigate the impact of zinc doping on the structure of akermanite, which was synthesized using the sol-gel combustion method, with the goal of improving the biological response. Powder XRD and FT-IR analysis confirmed the phase purity and the respective functional groups associated with Zn-doped akermanite.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!