We previously reported the sequencing of two genes (ndhA and ndhI) encoding two of the subunits of the type-I NADH-ubiquinone oxidoreductase from Rhodobacter capsulatus (Rc). The present paper deals with the cloning and characterization of a chromosomal fragment clustering five new Rc genes which encode subunits of this enzyme. This gene cluster is located immediately downstream from ndhA and ndhI, and also contains two unidentified open reading frames (urf2, urf3). The five genes, nuoJ, nuoK, nuoL, nuoM and nuoN, encode proteins related, respectively, to mitochondrial (mt) subunits ND6, ND4L, ND5, ND4 and ND2. The overall organization of the nuo genes identified in Rc shows similarity to that of the Paracoccus denitrificans (Pd) nqo gene cluster.
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Article Synopsis
- The PufX protein helps certain purple bacteria with the process of exchanging two important molecules, ubiquinol and ubiquinone, in their reaction centers where they convert light into energy.
- Scientists studied this process in a type of purple bacteria called Rhodobacter capsulatus, using a special technique to see what happens when the bacteria were illuminated with light.
- They found that in bacteria without the PufX protein, the reactions weren’t as efficient, meaning that the way the molecule structure was set up made it harder for the needed molecules to reach where they needed to be in the reaction center.
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