The equilibrium swelling of biopolymer loaded polyacrylamide gels can yield information on polymer-polymer interactions. Using the temperature dependence of excess equilibrium swelling, equations were derived to yield homopolymer as well as heteropolymer interactions. Hyaluronan and collagen (Type I) encapsulated in polyacrylamide gels showed increasing isothermal swelling with increase in the bipolymer concentration. The pH dependence of isothermal swelling caused by hyaluronan indicated that the swelling was largely due to repulsion of the charges of the carboxylate groups. Temperature dependent studies showed a decrease in swelling with an increase in temperature for collagen. The opposite trend was found for hyaluronan. This indicates that the interactions among collagen monomers are largely hydrophobic, while the interactions among hyaluronan molecules are mainly hydrogen bonding and repulsive ionic forces. Combined hyaluronan and collagen in polyacrylamide gels demonstrated an increase in swelling with an increase in temperature albeit much reduced compared with hyaluronan alone. Collagen interacts with hyaluronan through ionic attraction.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics.
J Proteome Res
January 2025
Advanced Research Support Center, Ehime University, Ehime 791-0295, Japan.
Precise prefractionation of proteome samples is a potent method for realizing in-depth analysis in top-down proteomics. PEPPI-MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation based on molecular weight by highly efficient extraction of proteins from polyacrylamide gels after SDS-PAGE separation. Thereafter it is essential to effectively remove contaminants such as CBB and SDS from the PEPPI fraction prior to mass spectrometry.
View Article and Find Full Text PDFPEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics.
Nat Protoc
January 2025
Advanced Research Support Center, Ehime University, Ehime, Japan.
Top-down analysis of intact proteins and middle-down analysis of proteins subjected to limited digestion require efficient detection of traces of proteoforms in samples, necessitating the reduction of sample complexity by thorough pre-fractionation of the proteome components in the sample. SDS-PAGE is a simple and inexpensive high-resolution protein-separation technique widely used in biochemical and molecular biology experiments. Although its effectiveness for sample preparation in bottom-up proteomics has been proven, establishing a method for highly efficient recovery of intact proteins from the gel matrix has long been a challenge for its implementation in top-down and middle-down proteomics.
View Article and Find Full Text PDFRole of 3D ultrasound in the treatment of stress urinary incontinence with polyacrylamide hydrogel (Bulkamid®) - A pilot study.
Eur J Obstet Gynecol Reprod Biol
February 2025
Department of Obstetrics and Gynecology, Aalborg University Hospital (AAUH), Denmark.
Introduction And Hypothesis: Polyacrylamide hydrogel (PAHG) is a bulking agent used in treatment of stress urinary incontinence (SUI) in women. Some women experience an improvement or cure in stress urinary incontinence (SUI) symptoms lasting only a few weeks after the injection of the bulking agent. The aim of this study was to use three-dimensional ultrasound (3DUS) to describe the changes in volume, number of deposits and echogenicity of the bulking agent 3 months after PAHG injection.
View Article and Find Full Text PDFEffect of polyacrylamide gel elasticity on collagen type II fibril assembly.
Soft Matter
December 2024
Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, OH, USA.
Collagen type II fibrils provide structural integrity to the articular cartilage extracellular matrix. However, the conditions that control the fibril radial size scale, distribution, and formation inside of dense networks are not well understood. We have investigated how surrounding elastic networks affect fibril formation by observing the structure and dynamics of collagen type II in model polyacrylamide gels of varying moduli.
View Article and Find Full Text PDFThe Effect of Different Concentrations of Melatonin on the Quality of Frozen and Thawed Semen of Jamnapari Goat.
Reprod Domest Anim
November 2024
Department of Veterinary Clinical Complex, West Bengal University of Animal & Fishery Sciences, Kolkata, India.
Article Synopsis
- Antioxidants protect sperm cells during freezing and thawing, with melatonin showing antioxidative effects.
- The study assessed the effects of melatonin at different concentrations (0.5, 1.0, and 2.0 mM) in tris-based extenders on Jamnapari goat sperm quality after freezing.
- Results indicated that melatonin improved sperm quality parameters, particularly at the 1.0 mM concentration, which was most effective in preventing cryodamage to the sperm cells.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!