AI Article Synopsis
- The study compared bilirubin measurements in neonates using Kodak Ektachem and Hitachi 717 Analysers along with HPLC methods, finding good agreement in total bilirubin but discrepancies in conjugated and direct bilirubin levels.
- A significant presence of configurational isomers was noted in all samples, while only a small percentage showed conjugated bilirubin or structural isomers.
- The development of a new device to produce specific isomers through light irradiation reveals that these isomers affect bilirubin readings, leading to potential inaccuracies in routine analyser results.
Article Abstract
We compared data obtained with the Kodak Ektachem and Hitachi 717 Analysers and HPLC from 83 neonates under phototherapy. Total bilirubin values determined with the Kodak and Hitachi are in good agreement, but we observed a large discrepancy in the results for conjugated (Kodak) and direct (Hitachi) bilirubin. HPLC revealed that all the samples contained configurational isomers, while only 7.7% and 30.8% contained conjugated bilirubin and structural isomers, respectively. We developed a device for the specific and quantitative production of configurational or structural isomers, by irradiation with blue or green light. In vitro, total bilirubin values are coherent for the routine analysers in the presence of configurational or structural isomers. With configurational isomers, unconjugated bilirubin (Kodak) is lower than total bilirubin (Kodak), and conjugated bilirubin (Kodak) is always equal to zero, so the apparatus gives a false positive response for delta bilirubin. In contrast, the direct bilirubin (Hitachi) is constant. Furthermore, in the presence of structural isomers, unconjugated bilirubin (Kodak) is unexpectedly higher than total bilirubin (Kodak), conjugated bilirubin (Kodak) is proportional to the quantity of these isomers, and direct bilirubin (Hitachi) is constant. The contribution of photoisomers in bilirubin measurements is discussed.
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| http://dx.doi.org/10.1515/cclm.1995.33.8.503 | DOI Listing |
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Article Synopsis
- The study compared bilirubin measurements in neonates using Kodak Ektachem and Hitachi 717 Analysers along with HPLC methods, finding good agreement in total bilirubin but discrepancies in conjugated and direct bilirubin levels.
- A significant presence of configurational isomers was noted in all samples, while only a small percentage showed conjugated bilirubin or structural isomers.
- The development of a new device to produce specific isomers through light irradiation reveals that these isomers affect bilirubin readings, leading to potential inaccuracies in routine analyser results.
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