AI Article Synopsis
- The study identifies intrachromosomal rearrangements at the 1p32 locus, linking the RLF gene to L-myc, and presents the full-length cDNA sequence of RLF mRNA, which is 6251 bp long.
- The RLF protein, consisting of 1914 amino acids and featuring sixteen zinc finger motifs, is related to the Zn-15 transcription factor and is expressed in various fetal and adult tissues, indicating its role in transcriptional regulation.
- The research suggests that these rearrangements may disrupt the regulation of the L-myc gene expression, even though the transforming ability of RLF-L-myc and normal L-myc proteins are similar.
Article Abstract
We have previously characterized intrachromosomal rearrangements at 1p32 fusing the first exon of the RLF gene with L-myc. Here we present the full-length cDNA sequence of the 6251 bp RLF mRNA. The predicted 1914 amino acid Rlf protein contains sixteen widely spaced zinc finger motifs, and is related to the Zn-15 transcription factor. RLF is widely expressed in fetal and adult tissues, suggesting that it has a general role in transcriptional regulation. The zinc fingers are not contained in the 79 amino acid N-terminal region of RLF involved in the RLF-L-myc fusions, and the transforming ability of the RLF-L-myc and the normal L-myc proteins is indistinguishable. These findings suggest that the role of the rearrangements fusing RLF and L-myc is to deregulate the tightly controlled expression of the L-myc gene.
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