Mol Biol (Mosk)
Published: February 1996
Chemistry
March 2025
University of Frankfurt, Cluster of Excellence Macromolecular Complexes, Max-von-Laue-Str. 9, 60438, Frankfurt, GERMANY.
We introduce a novel approach for the control of oligonucleotides through enzymatic activation with β-galactosidase (β-gal). We use the well-known enzymatic capability of β-gal to hydrolyze the β-galactosidic bond combined with a self-immolative linker and present three ways of sterical or topological blocking of a DNA oligonucleotide. Through a series of in vitro experiments utilizing β-gal variants (recombinant or from human cell lysates), we systematically investigate stability, transitory perturbation and enzymatic activation.
View Article and Find Full Text PDFMikrochim Acta
March 2025
School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, P. R. China.
The phosphorylation of nucleic acids mediated by 5'-polynucleotide kinase (PNK) exerts a crucial regulatory function in a wide range of significant cellular activities. Nevertheless, the current approaches for detecting PNK require expensive labeled probes and complex instrumentation, making it impossible to achieve real-time, on-site, and rapid analysis. Here, we take T4 PNK as a model and establish a novel colorimetric strategy for the detection of PNK activity and its inhibition by means of a coupled enzyme-assisted cyclic strand displacement amplification (SDA) and peptide nucleic acid (PNA)-gold nanoparticle (AuNP) based platform.
View Article and Find Full Text PDFScience
February 2025
National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Signaling from pathogen sensing to effector activation is a fundamental principle of cellular immunity. While cyclic (oligo)nucleotides have emerged as key signaling molecules, the existence of other messengers remains largely unexplored. Here, we reveal a bacterial anti-phage system that mediates immune signaling through nucleobase modification.
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February 2025
Department of Chemistry, Faculty of Science and Arts, Promising Centre for Sensors and Electronic Devices, Najran University, Najran, 11001, Kingdom of Saudi Arabia.
The development of a highly sensitive electrochemical aptasensor is reported that combines the unique properties of graphene, metallic oxides, and conducting polymers to overcome the limitations of sensitivity and specificity required for early diagnosis of Alzheimer's desease (AD). The sensor design utilizes a ternary composite of polypyrrole (PPy), reduced graphene oxide (rGO), and Fe₂O₃ nanoparticles, which synergistically enhance signal amplification, conductivity, and biocompatibility. The aptasensor leverages aptamers as biorecognition elements for the specific detection of Aβ1-40 oligomers.
View Article and Find Full Text PDFNat Commun
February 2025
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Republic of Korea.
Effective subset selection from complex oligonucleotide libraries is crucial for genomics, synthetic biology, and DNA data storage. The polymerase chain reaction, foundational for amplifying target subsets is limited by primer design and length for specificity, which constrains the scalability of oligo libraries and increases the synthesis burden for primers. We introduce an oligo subset selection methodology that utilizes sequence-specific cyclic nucleotide synthesis and blocking of the template oligos.
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