AI Article Synopsis
- Phosphoinositide-specific phospholipase C (PLC), particularly PLC-delta 1, plays a crucial role in signal transduction related to Alzheimer's disease (AD).
- An antibody targeting PLC-delta 1 revealed intense staining of neurofibrillary tangles in AD brains, indicating its association with the disease.
- Although PLC-delta 1 protein levels were higher in the cytosol of AD tissues, its activity was decreased, and both PLC-delta 1 and PLC-gamma 1 were found to be lower in the membranous fraction of AD brains compared to controls, suggesting unique roles of these isozymes in AD pathology.
Article Abstract
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. We demonstrated that an antibody to an isozyme of PLC, PLC-delta 1, produced intense staining of neurofibrillary tangles (NFT) in the brains of patients with Alzheimer's disease (AD). Western blot analysis using a specific antibody for PLC-delta 1 showed that the concentration of PLC-delta 1 protein was significantly higher in the cytosolic fraction of AD cortical tissues than in controls. The activity of PLC-delta 1, which hydrolyzes phosphatidylinositol, was not significantly different in the Alzheimer and control cytosolic fraction, indicating that the specific activity of PLC-delta 1 is decreased in Alzheimer brains. Western blot analysis also showed that the concentration of PLC-delta 1 and PLC-gamma 1 was significantly lower in the membraneous fraction of AD cortical tissues than in controls. These results suggest a differential involvement of PLC isozymes in AD.
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| http://dx.doi.org/10.1159/000213716 | DOI Listing |
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