Three minor peptaibols, trichokonins (TKs)-Ia, Ib, and IX, were obtained from the culture broth of Trichoderma koningii Oudemans. Primary structures of these peptaibols were elucidated by ion-spray ionization mass spectrometry (ISI-MS) including the collision-induced dissociation (CID) technique together with two-dimensional nuclear Overhauser enhancement spectroscopy (NOESY).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1248/cpb.43.1663 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sequences of Tolypins, Insecticidal Efrapeptin-Type Peptaibiotics from Species of the Fungal Genus Tolypocladium.
Chem Biodivers
July 2020
Interdisciplinary Research Centre for BioSystems, Land Use and Nutrition (IFZ), Department of Food Sciences, Institute of Nutritional Science, Justus-Liebig University of Giessen, Heinrich-Buff-Ring 26-32, DE, 35392 Giessen, Germany.
A peptide mixture named tolypin, originally isolated from species of the fungal genus Tolypocladium, was structurally characterised and sequences compared to those reported for efrapeptins isolated from strains of Tolypocladium inflatum. Chiral amino acid analysis, direct infusion, and online HPLC electrospray ionization tandem mass spectrometry provided composition, molecular weights of peptides, and series of diagnostic fragment ions. Sequences deduced from ESI-MS revealed that tolypins C-G are identical to efrapeptins C-G.
View Article and Find Full Text PDFNew Peptaibiotics and a Cyclodepsipeptide from : Isolation, Identification, Cytotoxic and Nematicidal Activities.
Antibiotics (Basel)
March 2020
Department of Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, , an antagonistic fungus previously isolated from the plant parasitic cyst nematode , was selected to carry out an in-depth metabolomic study for its active metabolites. Herein, three new nonapeptide peptaibols with leucinostatin based sequences were isolated and identified by 1, 2D NMR, and HR-ESI-MS-MS.
View Article and Find Full Text PDFHow are 1,2,3-triazoles accommodated in helical secondary structures?
Org Biomol Chem
May 2018
USR 3278 CRIOBE, PSL Research University, EPHE-UPVD-CNRS, Université de Perpignan Via Domitia, Laboratoire d'Excellence «CORAIL». Bâtiment T, 58 avenue P. Alduy, 66860 Perpignan, France.
1,4-Disubstituted-1,2,3-triazole (Tz) is widely used in peptides as a trans-amide bond mimic, despite having hazardous effects on the native peptide activity. The impact of amide bond substitution by Tz on peptide secondary structures is scarcely documented. We performed a Tz scan, by systematically replacing peptide bonds following the Aib residues with Tz on two model peptaibols: alamethicin F50/5 and bergofungin D, which adopt stable α- and 310 helices, respectively.
View Article and Find Full Text PDFOptimization of a UDP-glucuronosyltransferase assay for trout liver S9 fractions: activity enhancement by alamethicin, a pore-forming peptide.
Xenobiotica
December 2016
a United States Environmental Protection Agency (US EPA), ORD, NHEERL, Mid-Continent Ecology Division , Duluth , MN , USA.
1. An existing assay for UDP-glucuronosyltransferase (UGT) activity in trout liver microsomes was optimized using trout liver S9 fractions. Individual experiments were conducted to determine the time dependence of UGT activity as well as optimal levels of S9 protein, uridine 5'-diphosphoglucuronic acid (UDPGA), substrate (p-nitrophenol) and alamethicin, a pore-forming agent added to eliminate latency.
View Article and Find Full Text PDFThe sequences of the eleven-residue peptaibiotics: suzukacillins-B.
Chem Biodivers
May 2013
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources Project Group, Winchesterstrasse 2, D-35394 Giessen.
The filamentous fungus designated 'Trichoderma viride' strain 63 C-1 simultaneously produces suzukacillins (SZs), two microheterogeneous groups of peptaibols, under submerged culture conditions. Both groups are readily distinguishable by TLC: the major group is designated SZ-A, whereas the minor group with a higher Rf value is named SZ-B. The peptide mixture was obtained from a MeOH extract of the mycelium.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!