[The use of gnotobiological technology for producing and maintaining experimental, animals with different microbiological states of the intestines].

The standardization of all components ensures the optimum experimental conditions; for this reason the obtaining and maintenance of animals meeting GLP requirements is one of the development of scientific research in biology and medicine. The aim of the present work was the study of the possibility of using gnotobiological technology for obtaining animals with different microbial status of the intestine and their maintenance. Our investigations revealed that obtaining animals with different microbial status of the intestine and their maintenance under the conditions of total gnotobiological isolation (TGBI) prevented the contamination of the animals, thus facilitating the standardization of research procedures. Gnotobiological technology made it possible to create the optimum maintenance conditions and to ensure constant microbial status in animals, free from pathogenic flora. The maintenance to totally decontaminated mice under TGBI conditions ensured the total germ-free status in these animals for 9 days.