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[Examination of toxin production from environmental Bacillus cereus and Bacillus thuringiensis]. | LitMetric

AI Article Synopsis

  • The study analyzed 524 strains of Bacillus cereus and 90 strains of Bacillus thuringiensis to assess the presence of emetic and entero toxins in various environmental sources like food and soil.
  • Emetic toxin detection utilized an improved method involving HEp-2 cells, identifying that a limited number of Bacillus cereus strains from specific sources (bean paste and peach) produced this toxin, all categorized under the H1 serotype.
  • In contrast, Bacillus thuringiensis strains did not produce emetic toxin, but 47 of them were found to produce entero toxin.

Article Abstract

Emetic toxin- and entero toxin-producing activities were examined for 524 strains of Bacillus cereus and 90 strains of Bacillus thuringiensis to determine the distribution and contamination in natural environment, including foods, vegetable, soil and etc. Emetic toxin was assayed by the production of acid from HEp-2 cells induced by the culture supernatants of Bacillus, which was developed as an improved method for the detection of B. cereus heat-stable emetic-toxin by us. In the 524 strains of B. cereus tested, 11 out of 60 strains obtained from bean paste and 5 out of 20 strains obtained from peach produced emetic toxin which was detected by the HEp-2 cell method. All these emetic toxin-producing isolate strains were classified into H1 serotype. The 90 strains of B. thuringiensis did not produce emetic toxin, but the 47 strains produced entero toxin.

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Source
http://dx.doi.org/10.1248/yakushi1947.115.9_742DOI Listing

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