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http://dx.doi.org/10.1093/nar/21.10.2514 | DOI Listing |
Pharmacol Rep
June 2016
Dipartimento di Scienze Biomediche e Oncologia Umana, Università degli Studi di Bari, Ospedale Consorziale Policlinico, Bari, Italy. Electronic address:
Background: We describe the potentiation of antiproliferative and apoptotic activities triggered by cis-diamminedichloroplatinum(II) (DDP), and obtained in vitro by the co-administration of procainamide hydrochloride (PdHCl) in murine P388, and human A2780 and A549 cells.
Methods: We determined the antiproliferative and apoptotic activities of DDP and PdHCl combinations by different techniques. Moreover, cell cycle analysis, restriction enzyme inhibition followed by agarose gel electrophoresis, and TUNEL analysis of tumour cells in vivo were also used to strengthen our hypothesis.
J Virol Methods
December 2014
Shandong Key Laboratory of Preventive Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China. Electronic address:
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay has been developed to detect and differentiate between canine parvovirus (CPV) and mink enteritis virus (MEV). Eight CPV and three MEV epidemic strains isolated from 28 pathological samples from dogs and minks suspected of being infected with parvovirus were amplified by PCR using a pair of specific primers designed based on the CPV-N strain (M19296). PCR amplified a fragment of 1016bp from the genomic DNA of both MEV and CPV.
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