A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotype 5 and 8 capsular polysaccharides, was compared with three commercially available rapid agglutination methods for the identification of 220 isolates of S. aureus (61 oxacillin resistant) and 128 isolates of coagulase-negative staphylococci. The sensitivity for identification of S. aureus was high with the Pastorex Staph-Plus test (98.6%) compared with those of the other tests, which ranged from 91.8 to 84.5%. Test sensitivities for the identification of oxacillin-resistant S. aureus were as follows: Pastorex Staph-Plus, 95.1%; Pastorex Staph, 73.8%; Staphyslide, 72.1%; and StaphAurex, 49.2%.
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http://dx.doi.org/10.1128/jcm.31.5.1342-1344.1993 | DOI Listing |
Int J Antimicrob Agents
May 2024
Department of Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, Austria. Electronic address:
Introduction: Peritonitis remains the major infectious complication in the setting of peritoneal dialysis (PD). Despite known only moderate pathogenicity, the most frequently detected pathogens in PD-related peritonitis are surprisingly coagulase-negative staphylococci. However, this could be explained, at least in part, by Staphylococcus aureus small colony variants (SCVs) induced by PD fluids (PDFs) and misidentified by routinely used microbiological methods.
View Article and Find Full Text PDFBMC Oral Health
October 2019
Department of Oral Microbiology, Faculty of Medicine, Medical University of Gdansk, 25 Dębowa St, 80-204, Gdansk, Poland.
Background: The small-colony variants (SCVs) of Staphylococcus aureus were isolated from persistent and recurrent infections, especially after placement of medical devices having direct contact with human tissues. The emergence of SCVs is a survival strategy of S. aureus which enables them to hide inside host's cells and induces a less severe immune response than to wild-type S.
View Article and Find Full Text PDFPrzegl Epidemiol
December 2019
Department of Medical Microbiology, Medical University of Warsaw; T. Chalubinskiego 5 Str., 02-004 Warsaw, Poland
Introduction: Staphylococcus aureus is a microorganism, which is able to colonize the human body without any pathogenic effect, but it also can cause life-threatening infections (opportunistic pathogen). Asymptomatic colonization with both methicillin resistant (MRSA) and methicillin susceptible (MSSA) S.aureus strains state is an important predisposing factor for infections.
View Article and Find Full Text PDFPan Afr Med J
August 2015
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Democratic Republic of Congo.
Introduction: Surgical site infections (SSIs) after surgery are usually caused by Staphylococcus aureus and coagulase-negative staphylococci (CNS). In low income countries, methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant coagulase-negative staphylococci (MR-CNS) surgical site infections are particularly associated with high treatment cost and remain a source of mortality and morbidity. This study aimed to determine the prevalence and the sensitivity to antibiotics of MRSA and MR-CNS isolated from SSIs.
View Article and Find Full Text PDFJ Clin Microbiol
September 2014
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany
Rapid diagnosis is essential for the management of Staphylococcus aureus infections. A host recognition protein from S. aureus bacteriophage phiSLT was recombinantly produced and used to coat streptavidin latex beads to develop a latex agglutination test (LAT).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!