Background: Surface microscopy (SM) opened a new dimension in the clinical assessment of cutaneous pigmented lesions. Diagnostic patterns were described to provide guidelines for the preoperative diagnosis of pigmented skin lesions.
Objective: Our purpose was to explore whether "amplified" surface microscopy (ASM), by increasing magnification up to 400-fold, provides any improvement in the analysis of patterns previously described in cutaneous pigmented lesions.
Methods: A fiber-optic camera, allowing magnification up to 400 times and associated with an imaging system computer, was used for the analysis of 40 melanocytic lesions. Special emphasis was put on the most important features that can be observed in surface microscopy, the so-called pigment network and brown globules.
Results: The lines (grids) of the pigment network appeared to be composed of three zones: two darker external lines and one central clearer zone. This pattern, not previously seen with SM, is compatible with the anatomy of pigment accumulation in the epidermal rete ridges. Two types of brown globules could be distinguished: one located in the center of the pigment network holes, the other on the lines of its grids. The "on-the-grid" type was smaller than the "in-the-hole" type; it could be misinterpreted as "black dots" when seen at lower magnification (x10).
Conclusion: ASM is a tedious procedure that may help in the analysis of the features previously recognized by SM; SM is more accessible for routine use.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0190-9622(93)70131-c | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Facet-controlled electrosynthesis of nanoparticles by combinatorial screening in scanning electrochemical cell microscopy.
Nanoscale
January 2025
Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
Controlled synthesis of faceted nanoparticles on surfaces without explicit use of ligands has gained attention due to their promising applications in electrocatalysis and chemical sensing. Electrodeposition is a desirable method; however, precise control over their size, spatial distribution, and morphology requires extensive optimization. Here, we report the spatially resolved synthesis of shape-controlled Pt nanoparticles and fast screening of synthesis conditions in scanning electrochemical cell microscopy (SECCM) with pulse potentials.
View Article and Find Full Text PDFHeterogeneous Frustrated Lewis Pair Catalysts: Rational Structure Design and Mechanistic Elucidation Based on Intrinsic Properties of Supports.
Acc Chem Res
January 2025
The Wolfson Catalysis Centre, Department of Chemistry, University of Oxford, Oxford OX1 3QR, U.K.
ConspectusThe discovery of reversible hydrogenation using metal-free phosphoborate species in 2006 marked the official advent of frustrated Lewis pair (FLP) chemistry. This breakthrough revolutionized homogeneous catalysis approaches and paved the way for innovative catalytic strategies. The unique reactivity of FLPs is attributed to the Lewis base (LB) and Lewis acid (LA) sites either in spatial separation or in equilibrium, which actively react with molecules.
View Article and Find Full Text PDFA novel polysaccharide in the envelope of influences the septal secretion of preproteins with a YSIRK/GXXS motif.
J Bacteriol
January 2025
Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Unlabelled: Bacteria transport proteins across the plasma membrane to assemble their envelope, acquire nutrients, and establish appropriate interactions with their environment. The majority of these proteins are synthesized as precursors with a cleavable N-terminal signal sequence for recognition by the Sec machinery. In , a small subset of secreted precursors carries a YSIRK/GXXS motif.
View Article and Find Full Text PDFc-di-GMP phosphodiesterase ProE interacts with quorum sensing protein PqsE to promote pyocyanin production in .
mSphere
January 2025
School of Medicine, Southern University of Science and Technology, Shenzhen, China.
The universal bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) plays critical roles in regulating a variety of bacterial functions such as biofilm formation and virulence. The metabolism of c-di-GMP is inversely controlled by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs). Recently, increasing studies suggested that the protein-protein interactions between DGCs/PDEs and their partners appear to be a common way to achieve specific regulation.
View Article and Find Full Text PDFUse of analytical strategies to understand spatial chemical variation in bacterial surface communities.
J Bacteriol
January 2025
Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
Not only do surface-growing microbes such as biofilms display specific traits compared to planktonic cells, but also they display many heterogeneous behaviors over many spatial and temporal contexts. While the application of molecular genetics tools to extract or visualize gene expression or regulatory function data is now common in studying surface growth, the use of analytical chemistry tools to visualize the spatiotemporal distribution of chemical products synthesized by these surface microbes is less common. Here, we review chemical imaging tools that have been used to inform our understanding of surface-growing microbes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!