A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalga Porphyra yezoensis.
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