Double-stranded covalently closed circular supercoiled DNA (ccc DNA) from plasmid pUK 9 was irradiated in vitro at defined wavelengths in the UV region (290, 313 and 365 nm). The nicking was monitored by electrophoresis on agarose gels, ethidium staining and densitometric quantitation of supercoiled and relaxed moieties. At the explored wavelengths, the dose required for introducing one nick per million phosphodiester bonds diminishes with increased concentration of added ferric iron, whereas the effect of cupric iron is practically negligible. Adding metal chelators or bubbling argon prior to the irradiation results in a dramatic increase in the dose required for introducing one nick per million phosphodiester bonds. Taken together, these results seem to indicate that iron and oxygen play a role as cofactors in the UV-induced nicking of ccc DNA in vitro.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1751-1097.1993.tb02327.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sulfone-Embedded NIR Fluorophore with Large Stokes Shift for Monitoring Viscosity Changes during NAFLD-Induced Ferroptosis.
ACS Sens
January 2025
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China.
The accumulation of lipids in hepatocytes in nonalcoholic fatty liver disease (NAFLD) leads to an increase in reactive oxygen species and changes in the intracellular microenvironment, while ferroptosis is the result of the accumulation of iron-dependent lipid peroxidation. Studies have shown that ferroptosis plays an important role in the pathogenesis of NAFLD. Herein, we have developed a viscosity-sensitive fluorescence probe PTSO with near-infrared emission and a large Stokes shift, which were achieved by introducing the sulfone group into the dioxothiochromen-malononitrile fluorophore as an electron-withdrawing group.
View Article and Find Full Text PDFNovel enhancement strategy for Hg adsorption in wastewater: Nonthermal plasma-mediated advanced modification of zero-valent iron-carbon galvanic cells with thiol functionalization.
J Environ Manage
January 2025
Interdisciplinary Research Center for Construction and Building Materials, Department of Materials Science and Engineering, King Fahd University of Petroleum & Minerals, Dhahran, 31261, Saudi Arabia. Electronic address:
Mercury (Hg) pollution poses a critical threat to human health and the environment, necessitating urgent control measures. This study introduces a novel modification method for the common zero-valent iron-carbon (ZVI-AC) galvanic cells using a two-step process, nonthermal (NTP) irradiation followed by targeted functionalization, aiming to enhance Hg adsorption potential by adjusting the physicochemical properties of the cells. The NTP irradiated functionalized adsorbent demonstrated superior Hg adsorption performance across various concentrations and pH variations.
View Article and Find Full Text PDFSS-31@Fer-1 Alleviates ferroptosis in hypoxia/reoxygenation cardiomyocytes via mitochondrial targeting.
Biomed Pharmacother
January 2025
Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China. Electronic address:
Purpose: Targeting mitochondrial ferroptosis presents a promising strategy for mitigating myocardial ischemia-reperfusion (I/R) injury. This study aims to evaluate the efficacy of the mitochondrial-targeted ferroptosis inhibitor SS-31@Fer-1 (elamipretide@ferrostatin1) in reducing myocardial I/R injury.
Methods: SS-31@Fer-1 was synthesized and applied to H9C2 cells subjected to hypoxia/reoxygenation (H/R) to assess its protective effects.
Enhanced oxygen evolution reaction through improved lattice oxygen activity via carbon dots incorporation into MOFs.
J Colloid Interface Sci
January 2025
School of Materials Science and Engineering, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan 570228, China. Electronic address:
Emerging of the lattice oxygen mechanism (LOM) provides a new opportunity for enhancing oxygen evolution reaction (OER) activity. However, its stability suffers from metal cation dissolution and lattice oxygen anionic redox chemistry. In this paper, carbon dots (CDs)-modified nickel-iron MOF (Metal-Organic Framework) nanosheets (NiFe-BDC/CDs) were prepared for efficient OER electrocatalysis.
View Article and Find Full Text PDFCerium oxide nanoparticles decorated on graphene oxide nanosheets as battery-type cathode material for supercapacitors.
J Colloid Interface Sci
January 2025
Department of Physics, Malaviya National Institute of Technology Jaipur, Rajasthan 302017 India. Electronic address:
Designing advanced materials that effectively mitigate the poor cycle life of battery-type electrodes with high specific capacities is crucial for next-generation energy storage systems. Herein, graphene oxide-ceria (GO-CeO) nanocomposite synthesized via a facile wet chemical route is explored as cathode for high-performance supercapacitors. The morphological analysis suggests fine ceria (CeO) nanoparticles dispersed over ultrathin graphene oxide (GO) sheets while structural studies reveal face-centered cubic phase of CeO in the nanocomposite.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!