A 2.0-kb fragment from the Lactobacillus gasseri temperate bacteriophage phi adh contained the essential genetic determinants for site-specific integration. The nucleotide sequence of this fragment was determined. An open reading frame (intG), which adjoined the phage attachment site (attP), encoded a deduced protein related to the integrase family. The organization of this region was comparable to other phage site-specific recombination systems.
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http://dx.doi.org/10.1016/0378-1119(93)90590-y | DOI Listing |
Microorganisms
January 2025
Unidad de Investigación en Salud, Hospital de Alta Especialidad Ixtapaluca, IMSS-Bienestar, Carr Federal México-Puebla Km 34.5, Ixtapaluca 56530, Mexico.
Unlabelled: Infertility, both primary and secondary, is strongly influenced by microbiological factors, with the vaginal microbiota playing a key role in reproductive health.
Objective: The aim of this study was to characterize the vaginal microbiota of 136 Mexican women diagnosed with infertility-primary (n = 58) and secondary (n = 78)-by evaluating the presence of pathogenic bacterial species and their associations with infertility conditions.
Methods: Samples were obtained through cervical swabs, and microorganism identification was performed using qPCR techniques.
Europace
January 2025
Department of Cardiology, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
Ibrutinib, a widely used anti-cancer drug, is known to significantly increase the susceptibility to atrial fibrillation (AF). While it is recognized that drugs can reshape the gut microbiota, influencing both therapeutic effectiveness and adverse events, the role of gut microbiota in ibrutinib-induced AF remains largely unexplored. Utilizing 16S rRNA gene sequencing, fecal microbiota transplantation, metabonomics, electrophysiological examination, and molecular biology methodologies, we sought to validate the hypothesis that gut microbiota dysbiosis promotes ibrutinib-associated AF and to elucidate the underlying mechanisms.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Engineering, China Agricultural University, Beijing 100083, China. Electronic address:
Bacteriocins, naturally derived antimicrobial peptides, are considered promising alternatives to traditional preservatives and antibiotics, particularly in food and medical applications. Despite extensive research on various bacteriocins, cyclic varieties remain understudied. This study introduces Gassericin GA-3.
View Article and Find Full Text PDFJ Control Release
December 2024
Major of Human Bio-convergence, Division of Smart Healthcare, Pukyong National University, Busan 48513, Republic of Korea. Electronic address:
Inflammatory bowel disease (IBD) is a chronic or recurrent inflammatory disorder affecting various parts of the gastrointestinal tract. Metformin, a widely prescribed hypoglycemic drug for type 2 diabetes, has shown potential in reducing IBD risk. However, its oral administration faces significant challenges due to the harsh gastrointestinal environment, requiring higher or more frequent doses to achieve therapeutic effects, which increases the risk of side effects.
View Article and Find Full Text PDFContemp Clin Trials
December 2024
UCD Perinatal Research Centre, UCD School of Medicine, University College Dublin, National Maternity Hospital, Dublin 2, Ireland; National Maternity Hospital, Dublin 2, Ireland. Electronic address:
Introduction: Effective spontaneous preterm birth (sPTB) prevention is an urgent unmet clinical need. Vaginal depletion of Lactobacillus crispatus is linked to sPTB. This trial will investigate impact of an oral Lactobacillus spp.
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