AI Article Synopsis
- The study compared various measurements related to nuclear weight, chromatin fiber properties, and DNA amounts in lymphocytes from humans, chickens, frogs, and trout.
- Results showed that frogs had the highest nuclear mass and DNA amounts, while chickens had the least, and fiber diameter was largest in trout, followed by chickens, frogs, and humans.
- The "packing ratio" of DNA in chromatin fibers varied among species, indicating lower ratios in amniotes (like chickens and humans) compared to anamniotes (like frogs and trout), but no evolutionary conclusions could be drawn due to the limited sample size.
Article Abstract
1. Quantitative electron microscopic and cytophotometric determinations of average nuclear weight, unit weight of chromatin fiber, chromatin fiber diameter and DNA amount were made and compared from lymphocytes in human, chicken, frog and trout. 2. From these determinations the "packing ratio" of DNA in chromatin fibers was calculated. 3. Nuclear mass and DNA amounts were highest in frog and lowest in chicken, while fiber diameter and mass of unit fiber measured highest in trout followed by chicken, frog and man. 4. The packing ratios were 21.4 in chicken, 35.2 in human, 47.0 in trout, 51.1 in frog and show a trend of being lower in amniotes than in anamniotes. 5. As only four species were studied, no conclusions regarding evolutionary implications or relationships between them could be made from these measurements.
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| http://dx.doi.org/10.1016/0305-0491(93)90207-l | DOI Listing |
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