AI Article Synopsis
- The study examined how different concentrations of sodium nitrite (NaNO2) influenced various metabolic processes in isolated rat liver cells, focusing on 15N-ammonia utilization, glucose, pyruvate, lactate production, and glycogen breakdown.
- Nitrite didn't change glycogen breakdown or lactate/pyruvate formation but significantly boosted glucose production, indicating enhanced gluconeogenesis triggered by nitrite.
- Higher nitrite levels led to reduced ammonia use and urea production, showing strong negative correlations with nitrite concentration, suggesting that nitrite directly disrupts nitrogen and carbohydrate metabolism.
Article Abstract
The effect of three NaNO2 concentrations (0.5, 2.0, and 5.0 mM) on 15N-ammonia utilization, ureagenesis, glucose, pyruvate and lactate formation and glycogen breakdown were studied in isolated rat hepatocytes. Nitrite failed to affect the rate of glycogenolysis as well as the lactate and pyruvate formation, but at the same time it markedly increased the glucose formation. It is concluded that the increase in the glucose formation results from the nitrite stimulation of the rate of gluconeogenesis. An increased sodium nitrite concentration caused a significant decrease in the ammonia utilization and urea synthesis; there are strong linear correlations between the nitrite concentration and the amount of utilized ammonia (r = -0.93) and the formed urea (r = -0.96). The observed lower rate of ureagenesis in the presence of nitrite resulted from the diminished incorporation of the added 15N-ammonia into urea, as well as from the diminished urea formation from endogenous nitrogen. It is concluded that the disturbances in carbohydrate and nitrogen metabolism observed in the nitrite-poisoned animals are attributed to the direct effect of nitrite on metabolism.
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| http://dx.doi.org/10.1007/BF01128737 | DOI Listing |
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