A simple and rapid high-performance liquid chromatographic method with UV detection for the determination in serum of the antimalarial artelinic acid is described. Artelinic acid is a water-soluble semi-synthetic derivative of artemisinin (or Qinghaosu), which is the parent compound of a completely new class of antimalarials with great potential value. Calibration curves of artelinic acid in serum from 0.5 to 50 micrograms/ml were found to be linear, and the extraction recovery was ca. 70% from rabbit serum and 45% from bovine and human serum. The detection limit of the method was 50 ng/ml when 250 microliters of serum were processed. Spiked samples (rabbit serum) proved to be stable for at least 43 days on storage at -20 degrees C. The method has been used for the analysis of ca. 400 samples in a pharmacokinetic study. About 50 samples can be processed in half a day.
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http://dx.doi.org/10.1016/0378-4347(93)80182-4 | DOI Listing |
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