AI Article Synopsis
Article Abstract
A new form of TdT mRNA has been identified by screening a mouse thymus cDNA library. It contains an open reading frame of 1527 base pairs corresponding to a protein containing 509 aminoacids, whereas the previously identified mouse TdT mRNA is composed of 1587 base pairs and encodes a protein of 529 aminoacids. Analysis of a mouse genomic clone containing the 3' portion of the TdT gene shows that these twenty additional aminoacids are encoded by an additional exon located between exons X and XI. Both forms of TdT mRNA are present in the thymus and could be generated by alternative splicing. The cDNA reported here corresponds to the major form of TdT mRNA in Balb/c mice and closely resembles human and bovine TdT cDNA. Expression of this cDNA in mammalian cells shows that it encodes a functional protein capable of catalysing N region insertions at the recombination junction of an episomic recombination substrate.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC309280 | PMC |
| http://dx.doi.org/10.1093/nar/21.5.1187 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
METTL14 Promotes Lipopolysaccharide-Induced Myocardial Damage via m6A-Dependent Stabilization of TRPM7 mRNA.
Int Heart J
December 2024
Department of Pediatrics, Puren Hospital, Wuhan University of Science and Technology.
Article Synopsis
- Sepsis-induced myocardial injury (SIMI) is crucial in severe sepsis and septic shock, with METTL14 playing a key role in m6A RNA modification related to cardiac cell injury.
- LPS treatment leads to reduced cell viability and increased apoptosis and oxidative stress in human cardiomyocyte cells, with METTL14 and TRPM7 being upregulated.
- METTL14 enhances TRPM7 mRNA stability through m6A methylation; knockdown of METTL14 mitigates LPS-induced cardiac damage, suggesting a potential treatment target for septic cardiomyopathy.
Geraniin attenuates isoproterenol-induced cardiac hypertrophy by inhibiting inflammation, oxidative stress and cellular apoptosis.
Korean J Physiol Pharmacol
November 2024
Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
Article Synopsis
- Geraniin, a polyphenol found in fruit peel, exhibits anti-inflammatory and antioxidant properties that may protect the heart from damage.
- In a study, mice were treated with geraniin or spironolactone before being exposed to isoproterenol, which typically induces cardiac hypertrophy.
- Results showed that geraniin reduced heart remodeling, inflammation, and oxidative stress, suggesting its potential as a therapeutic agent against heart enlargement and damage.
Indirubin induces apoptosis in ovarian cancer cells via the mitochondrial pathway.
Am J Transl Res
October 2024
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University Jinan 250012, Shandong, China.
Objective: To investigate the pro-apoptotic effects of Indirubin, a traditional Chinese medicine, on ovarian cancer SKOV3 cell line and to explore its underlying mechanisms.
Methods: Ovarian cancer SKOV3 cells were divided into a control group (cells cultured normally) and an experimental group (cells cultured in medium containing Indirubin). SKOV3 cells at the logarithmic phase were treated with Indirubin at various concentrations.
Dexmedetomidine Alleviates the Long-Term Neurodevelopmental Toxicity Induced by Sevoflurane in the Developing Brain.
Dev Neurosci
October 2024
The Department of Clinical Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin, China.
Introduction: Sevoflurane is an extensively used anesthetic for pediatric patients; however, numerous studies showed that sevoflurane (SEVO) may cause long-term neurodevelopmental toxicity. Dexmedetomidine (DEX) has been shown to be protective against SEVO-induced neurotoxicity, but the mechanism remains unclear. The effects and mechanisms of different DEX administration routes on SEVO-induced neurotoxicity and long-term cognitive defects were determined and further investigated the role of sex in these processes.
View Article and Find Full Text PDFTCF4 as a potential prognostic biomarker and an anticancer target in gastric cancer.
Transl Cancer Res
September 2024
Department of Laboratory Medicine, Maternal and Child Health Care Hospital of Linhe District, Bayannaoer City, China.
Background: Lymphoid enhancer-binding factor 1 (LEF1)/T cell factor (TCF) family members are key transcription factors in malignant tumors. In this study, the role of T cell factor 4 (TCF4) in the progression of gastric cancer (GC) cell migration and invasion was investigated.
Methods: Fifty-five pairs of GC tissues and adjacent non-tumor tissues were collected for evaluating the expression of LEF1/TCF family members, which were also evaluated by the Gene Expression Profiling Interactive Analysis (GEPIA) database, an online analysis platform based on The Cancer Genome Atlas and Genotype-Tissue Expression databases.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!