A theoretical model is described that predicts that individuals with a glucose-6-phosphate dehydrogenase deficiency may experience acute hemolysis on exposure to ozone at levels reached in certain urban centers.
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http://dx.doi.org/10.1080/15287397709529470 | DOI Listing |
Protein Expr Purif
January 2025
Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, 131 Princess St, Manchester, M1 7DN, United Kingdom. Electronic address:
Since their discovery in Mycobacterium tuberculosis (Mtb), F-dependent enzymes have been identified as both important drug targets and potential industrial biocatalysts, including for bioremediation of otherwise recalcitrant substrates. Mtb-FGD1, utilizes glucose 6-phosphate (G6P) as an electron donor for the reduction of F. Current expression systems for Mtb-FGD1 use Mycobacterium smegmatis as host, because of the tendency for it to form inclusion bodies in E.
View Article and Find Full Text PDFNat Metab
January 2025
State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
Nucleotide availability is crucial for DNA replication and repair; however, the coordinating mechanisms in vivo remain unclear. Here, we show that the circadian clock in the liver controls the activity of the pentose phosphate pathway (PPP) to support de novo nucleotide biosynthesis for DNA synthesis demands. We demonstrate that disrupting the hepatic clock by genetic manipulation or mistimed feeding impairs PPP activity in male mice, leading to nucleotide imbalance.
View Article and Find Full Text PDFCurr Biol
January 2025
Institute for Microbiology, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany. Electronic address:
Non-N-fixing cyanobacteria enter a state of dormancy when fixed nitrogen becomes limiting. Resuscitation from this state involves a complex program of events. A new study reveals how the dormancy-resuscitation switch is governed by metabolite-level control of glucose-6-phosphate dehydrogenase activity.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
We developed a single-molecule enzyme activity assay platform for NAD(P)-dependent oxidoreductases, leveraging a new NAD(P)H-responsive fluorogenic probe optimized for microdevice-based fluorometric detection. This platform enabled the detection of enzyme activities in blood and cerebrospinal fluid (CSF), including lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and hexokinases. We demonstrate its potential for activity-based diagnosis by detecting altered populations of enzyme activity species in blood and CSF from liver damage in brain tumor patients.
View Article and Find Full Text PDFBr J Haematol
January 2025
Département de Biochimie-Pharmacologie, APHP (Assistance Publique-Hôpitaux de Paris), Hôpitaux Universitaires Henri Mondor, Créteil, France.
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