The influence of beta-propiolactone action on the immunogenic and protective activity of the influenza virus A/WSN/33 (H1N1) has been studied. The production of antibodies against virion surface antigens in mice immunized intramuscularly by the modified virus was enhanced with the increase of inoculating dose from 6 x 10(7) to 1.5 x 10(8) viral particles per animal. The immunizing dose of 6 x 10(7) produced complete protection of immunized animals against a lethal challenge of A/WSN/33 virus. The inhibition of virus reproduction in animal lungs was increased with the increase of the virus immunizing dose up to 6 x 10(8). At a constant dose the inhibition of virus reproduction decreases with the increase of the virus modification extent.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0264-410x(93)90197-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a high-performance liquid chromatography using rhodamine B hydrazide as the derivatization reagent for determination of β propiolactone residues in inactivated COVID-19 vaccines.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2024
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; PharmaBridge, Pharmaceutical Consultancy Company, Jule Stynestraat 102 3543 DS, Utrecht, the Netherlands. Electronic address:
Article Synopsis
- * A new High-Performance Liquid Chromatography-Ultraviolet (HPLC-UV) method was developed using Rhodamine B hydrazide (RBH) as a derivatizing agent, optimized in weak acidic conditions, and successfully separated on a C8 column.
- * The method showed high linearity (r2 > 0.99), low detection limits (0.17 µg/mL), and was applied to analyze BPL residues in inactivated COVID-19 vaccines, promising for quality control and regulatory
Sample-Treatment with the Virucidal β-Propiolactone Does Not Preclude Analysis by Large Panel Affinity Proteomics, Including the Discovery of Biomarker Candidates.
Anal Chem
June 2024
Institute of Translational Proteomics, Biochemical/Pharmacological Centre, Philipps-Universität Marburg, 35043 Marburg, Germany.
Virus inactivation is a prerequisite for safe handling of high-risk infectious samples. β-Propiolactone (BPL) is an established reagent with proven virucidal efficacy. BPL primarily reacts with DNA, RNA, and amino acids.
View Article and Find Full Text PDFCopolymerization of β-Butyrolactones into Functionalized Polyhydroxyalkanoates Using Aluminum Catalysts: Influence of the Initiator in the Ring-Opening Polymerization Mechanism.
Macromol Rapid Commun
July 2024
Departamento de Química Orgánica y Química Inorgánica, Instituto de Investigación en Química "Andrés M. del Río" (IQAR), Universidad de Alcalá, Campus Universitario, Alcalá de Henares, Madrid, 28871, Spain.
Within bioplastics, natural poly(3-hydroxybutyrate) (PHB) stands out as fully biocompatible and biodegradable, even in marine environments; however, its high isotacticity and crystallinity limits its mechanical properties and hence its applications. PHB can also be synthesized with different tacticities via a catalytic ring-opening polymerization (ROP) of rac-β-butyrolactone (BBL), paving the way to PHB with better thermomechanical and processability properties. In this work, the catalyst family is extended based on aluminum phenoxy-imine methyl catalyst [AlMeL], that reveals efficient in the ROP of BBL, to the halogeno analogous complex [AlClL].
View Article and Find Full Text PDFAn inactivated whole-virion vaccine for Enterovirus D68 adjuvanted with CpG ODN or AddaVax elicits potent protective immunity in mice.
Vaccine
April 2024
Laboratory of Nano-design for Innovative Drug Development, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan; Vaccine Creation Group, BIKEN Innovative Vaccine Research Alliance Laboratories, Research Institute for Microbial Diseases, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan; The Research Foundation for Microbial Diseases of Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Vaccine Creation Group, BIKEN Innovative Vaccine Research Alliance Laboratories, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Center for Advanced Modalities and DDS, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Global Center for Medical Engineering and Informatics, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Center for Infectious Disease Education and Research, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:
Enterovirus D68 (EV-D68), a pathogen that causes respiratory symptoms, mainly in children, has been implicated in acute flaccid myelitis, which is a poliomyelitis-like paralysis. Currently, there are no licensed vaccines or treatments for EV-D68 infections. Here, we investigated the optimal viral inactivation reagents, vaccine adjuvants, and route of vaccination in mice to optimize an inactivated whole-virion (WV) vaccine against EV-D68.
View Article and Find Full Text PDFEfficacy of an inactivated influenza vaccine adjuvanted with Toll-like receptor ligands against transmission of H9N2 avian influenza virus in chickens.
Vet Immunol Immunopathol
February 2024
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address:
Avian influenza viruses (AIV), including the H9N2 subtype, pose a major threat to the poultry industry as well as to human health. Although vaccination provides a protective control measure, its effect on transmission remains uncertain in chickens. The objective of the present study was to investigate the efficacy of beta-propiolactone (BPL) whole inactivated H9N2 virus (WIV) vaccine either alone or in combination with CpG ODN 2007 (CpG), poly(I:C) or AddaVax™ (ADD) to prevent H9N2 AIV transmission in chickens.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!