AI Article Synopsis
- Intracisternal A-particle (IAP) elements are repeated and spread throughout the mouse genome, particularly in normal B- and T-cells.
- Specific oligonucleotide probes target three active IAP subfamilies, identifying limited restriction fragments in DNA analysis.
- By comparing strain distribution patterns of these fragments with known genetic markers, researchers have mapped 44 proviral loci, contributing valuable markers to the mouse genome map.
Article Abstract
Intracisternal A-particle (IAP) proviral elements are moderately reiterated and widely dispersed in the mouse genome. Oligonucleotide probes have been derived from three distinctive IAP element subfamilies (LS elements) that are transcriptionally active in normal mouse B- and T-cells. In HindIII digests, LS element-specific oligonucleotides each react with a limited number of restriction fragments that represent junctions between proviral and flanking DNA. These fragments have characteristic strain distribution patterns (SDPs) which are polymorphic in the DNAs of different mouse strains. We have established chromosomal assignments for 44 LS proviral loci by comparing their SDPs with those of known genetic markers in the BXD set of RI mouse strains. Some of the loci have also been scored in the CXB RI set. The IAP LS loci can provide a significant number of markers with a recognized genetic organization to the mouse genome map.
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| http://dx.doi.org/10.1007/BF00290429 | DOI Listing |
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