A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this organism. The library was constructed in the lambda GEM-11 phage system. After plaque purification and reprobing, positive recombinant phages were chosen for further analysis. Characterization of the cloned DNA by restriction mapping and Southern hybridization showed that a 1.5-kb SalI fragment hybridized to the probe. Polymerase chain reaction assays using this fragment as a template and the probe as a primer indicated that the fragment carries the entire putative CH gene (cmcI). This was confirmed through the expression of CH enzymatic activity when the fragment was introduced into Streptomyces lividans. A putative beta-lactamase activity was detected in S. lividans.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC187609 | PMC |
| http://dx.doi.org/10.1128/AAC.37.1.84 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of Small RNAs in Using High-Resolution Transcriptomics and Expression Profiling During Clavulanic Acid Production.
Int J Mol Sci
December 2024
Grupo de Investigación en Simulación, Diseño, Control y Optimización de Procesos (SIDCOP), Departamento de Ingeniería Química, Universidad de Antioquia, Medellín 050010, Colombia.
Small non-coding RNAs play a pivotal role in regulating various metabolic processes in both prokaryotic and eukaryotic organisms. However, knowledge about small RNAs (sRNAs) in () is scarce. This study aimed to use cutting-edge bioinformatics tools and a compendium of RNA-seq data to predict the potential coding of sRNAs that might be present in the genome of ATCC 27064.
View Article and Find Full Text PDFGenomic Diversity of : Implications for Clavulanic Acid Biosynthesis and Industrial Hyperproduction.
Int J Mol Sci
October 2024
Grupo de Investigación en Simulación, Diseño, Control y Optimización de Procesos (SIDCOP), Departamento de Ingeniería Química, Universidad de Antioquia, Medellín 050010, Colombia.
is a species used worldwide to industrially produce clavulanic acid (CA), a molecule that enhances antibiotic effectiveness against β-lactamase-producing bacterial strains. Despite its low inherent CA production, hyper-producing strains have been developed. However, genomic analyses specific to and CA biosynthesis are limited.
View Article and Find Full Text PDFStreptomyces yaizuensis sp. nov., a berninamycin C-producing actinomycete isolated from sponge.
J Antibiot (Tokyo)
January 2025
Department of Biotechnology, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Japan.
While screening for antibiotics in a marine sample, we discovered a berninamycin C-producing actinomycete, designated YSPA8, isolated from a sponge. A polyphasic approach was used to determine the taxonomic position of the strain. Strain YSPA8 formed sympodially branched aerial mycelia that ultimately segment into chains of spores.
View Article and Find Full Text PDFGlobal regulator AdpA directly binds to tunicamycin gene cluster and negatively regulates tunicamycin biosynthesis in Streptomyces clavuligerus.
World J Microbiol Biotechnol
October 2024
Department of Agricultural Biotechnology, Ondokuz Mayıs University, Atakum, Samsun, 55139, Türkiye.
Since a transcriptional regulator has yet to be identified within the tunicamycin biosynthetic gene cluster in Streptomyces clavuligerus, we conducted a comprehensive investigation by focusing on the possible function of the pleiotropic regulator AdpA on tunicamycin. The genes encoding early steps of tunicamycin biosynthesis were significantly upregulated in S. clavuligerus ΔadpA.
View Article and Find Full Text PDFEffect of Soy Protein Products on Growth and Metabolism of , , and .
Foods
May 2024
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
Microbial nitrogen sources are promising, and soy protein as a plant-based nitrogen source has absolute advantages in creating microbial culture medium in terms of renewability, eco-friendliness, and greater safety. Soy protein is rich in variety due to different extraction technologies and significantly different in the cell growth and metabolism of microorganisms as nitrogen source. Therefore, different soy proteins (soy meal powder, SMP; soy peptone, SP; soy protein concentrate, SPC; soy protein isolate, SPI; and soy protein hydrolysate, SPH) were used as nitrogen sources to culture , , and to evaluate the suitable soy nitrogen sources of the above strains.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!