AI Article Synopsis
- Manganese superoxide dismutase (MnSOD) cDNAs from various mouse organs have three sequence differences compared to the MnSOD cDNA from mouse placenta.
- These differences involve amino acid substitutions: Val(-7) changes to Gly and Met(114) changes to Val, along with a deletion in the 3' untranslated region.
- The study's findings can aid in verifying sequences of wild-type and may help identify potential point mutations.
Article Abstract
Manganese superoxide dismutase (MnSOD)-encoding cDNAs from multiple mouse organs, including liver, kidney, brain, spleen and heart, show three sequence differences compared with the previously published mouse placenta MnSOD cDNA. The differences cause substitutions of Val(-7)-->Gly (GTG-->GGT) and Met(114)-->Val (ATG-->GTG) and a G746 deletion in the 3' untranslated region. The analysis, by reverse transcriptase-polymerase chain reaction-direct sequencing, of BALB/c mouse placenta RNA revealed the same sequence in the placenta MnSOD as that from multiple mouse organs. The data presented will be useful for wild-type sequence verification and possible point mutation detection.
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| http://dx.doi.org/10.1016/0378-1119(93)90311-p | DOI Listing |
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