AI Article Synopsis
- DNA-binding protein extracts can be successfully obtained directly from liquid nitrogen-preserved tissue culture cells without needing to culture them again.
- The study involved extracting DNA-binding proteins from T47D, Jurkat, and CAC-L153S cell lines that had been stored for up to one year.
- The DNA binding activity of a repressor related to mouse mammary tumor virus transcription was found to be the same in extracts from frozen cells as in those from freshly cultured cells.
Article Abstract
We demonstrate that DNA-binding protein extracts can be effectively prepared directly from tissue culture cells preserved under liquid nitrogen without returning the cells to culture. We prepared DNA-binding protein extracts directly upon thawing of T47D, Jurkat and CAC-L153S cell lines after storage in liquid nitrogen for periods of up to one year. Our results show that DNA binding of a repressor of mouse mammary tumor virus transcription in these extracts is indistinguishable from binding activity in similar extracts prepared from cells maintained in culture.
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