AI Article Synopsis
- Chronic treatment of 3T3-L1 adipocytes with okadaic acid boosts deoxyglucose uptake by 25 times, peaking at a 35nM concentration.
- This effect correlates with a significant 21-fold increase in the mRNA expression of glucose transporter 1 (glut 1).
- The study examines these results in the context of glucose transporter regulation and insulin signaling, comparing them to earlier research on the immediate effects of okadaic acid on glucose transporter movement.
Article Abstract
We present evidence that chronic 24 hour treatment of 3T3-L1 adipocytes with the phosphatase inhibitor okadaic acid increases deoxyglucose uptake 25 fold with a maximal effect at a concentration of 35nM. This pharmacological response is associated with a 21 fold increase in expression of glucose transporter 1 (glut 1) mRNA. These findings are discussed with respect to glucose transporter gene regulation and insulin signalling and are compared to previous observations describing the acute effects of okadaic acid on glucose transporter translocation.
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| http://dx.doi.org/10.1006/bbrc.1993.2046 | DOI Listing |
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