A 3-kb fragment hybridizing with a bovine alpha-lactalbumin cDNA probe was isolated from a bovine genomic library and completely sequenced. An internal fragment beginning downstream from exon 2, as already reported for another pseudogene, but ending in the 3'-untranslated region of exon 4 shares 78% sequence similarity with the bovine alpha-lactalbumin gene. This region is flanked by two directly repeated LINE sequences. The 5' ends of the fragment and of the aforementioned pseudogene share a specific nucleotide stretch, which suggests that they might have had a common origin.
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http://dx.doi.org/10.1006/geno.1993.1223 | DOI Listing |
Anal Biochem
April 2025
Advanced Electrophoresis Solutions Ltd., 380 Jamieson Parkway, Unit 7 and 8, ON, N3C 4N4, Canada; AES Biotech Jiaxing Ltd., No. 501 South Changsheng Road, Economic and Technological Development Zone, Jiaxing City, Zhejiang Province, PR China. Electronic address:
Characterizing major bovine milk proteins, including whey and casein, is of significant interest in the dairy industry. The diverse array of protein proteoforms can be different in terms of genetic variation, breed ways, lactation stage, and animal nutritional status. Current routine methods for bovine milk protein profiling are typically based on immunological techniques, infrared spectroscopy, slab gel isoelectric focusing, capillary electrophoresis, and high-performance liquid chromatography.
View Article and Find Full Text PDFJ Agric Food Chem
January 2025
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Milk proteins possess an abundance of free amino groups and exhibit diverse spatial structures. During food processing, these properties facilitate their interaction with hydrophobic ligands, such as linolenic acid. Exploring the IgE and IgG binding ability of linolenic acid-milk protein complexes at different temperatures, times, and molar ratios is crucial for controlling the allergenicity of milk proteins in food processing.
View Article and Find Full Text PDFNutrients
November 2024
Department of Nutrition, University of California, Davis, CA 95616, USA.
Food Res Int
November 2024
Department of Food Science, College of Agricultural and Life Sciences, Cornell University, Ithaca, NY, 14853, USA. Electronic address:
Food Res Int
November 2024
STLO, L'Institut Agro, INRAE, 35042 Rennes, France. Electronic address:
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