AI Article Synopsis
- A DNA fragment with genes for phosphatidylcholine-specific phospholipase C and sphingomyelinase from Bacillus cereus VKM-B164 was cloned and sequenced using pUC19 plasmid.
- The sequenced genes were compared to those from other B. cereus strains, specifically SE-1, IAM-1208, and GP-4.
- The findings showed that the VKM-B164 strain's gene sequences for both enzymes matched those of the SE-1 strain but differed from the gene sequences in the GP-4 and IAM-1208 strains.
Article Abstract
A BamHI-EcoRV DNA fragment, containing tandemly arranged genes for the Bacillus cereus VKM-B164 phosphatidylcholine-specific phospholipase C and sphingomyelinase, has been cloned and sequenced in pUC19 plasmid (EMBL accession No X64141). The obtained gene sequences have been compared with those the B. cereus strains SE-1, IAM-1208 and GP-4 reported previously. Both phospholipase C and sphingomyelinase gene sequences of the VKM-B164 strain were found to be identical to those of the strain SE-1 and to differ from the sequences of GP-4 and IAM-1208 strains, containing another version of the gene sequences.
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