Crystallization and preliminary crystallographic studies of human beta-glucuronidase.

J Mol Biol

E. A. Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, MO 63104.

Published: September 1993

Crystals of human beta-glucuronidase have been obtained by the vapor diffusion method, using 2-methyl-2,4-pentanediol as a precipitant. The crystals belong to the orthorhombic space group P222(1), with cell dimensions a = 134.5 A, b = 95.1 A, c = 124.4 A. The unit cell contains two copies of the tetrameric enzyme. Complete native data have been collected to a resolution of 2.6 A.

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http://dx.doi.org/10.1006/jmbi.1993.1494DOI Listing

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