A rapid method for the determination of skatole (3-methylindole) and indole in adipose tissue of pigs by reversed-phase high-performance liquid chromatography has been developed. Tissue samples were melted in a microwave oven, and 100 microliters of the liquid fat were dissolved in 1 ml of n-hexane and extracted with acetonitrile-water (75:25, v/v). Portions of 100 microliters of the solution were used for chromatographic analysis. Elution was performed on a reversed-phase column with a mobile phase composed of acetic acid and isopropanol (70:30, v/v). A fluorescence detector was used for quantification. The detection limit was 4 ng/g fat. The mean recoveries of added amounts of skatole and indole were 98.9 and 93.8%, respectively. The mean coefficients of variation were: inter-assay, 6.6% (skatole) and 8.8% (indole); intra-assay, 4.2% (skatole) and 2.9% (indole). Mean skatole concentrations in fat samples from boars (40 ng/g; n = 349) were not significantly higher than those from barrows (24 ng/g; n = 98).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0378-4347(93)80387-j | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Direct Assembly of Grooved Micro/Nanofibrous Aerogel for High-Performance Thermal Insulation via Electrospinning.
ACS Appl Mater Interfaces
January 2025
CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Maintaining human body temperature in both high and low-temperature environments is fundamental to human survival, necessitating high-performance thermal insulation materials to prevent heat exchange with the external environment. Currently, most fibrous thermal insulation materials are characterized by large weight, suboptimal thermal insulation, and inferior mechanical and waterproof performance, thereby limiting their effectiveness in providing thermal protection for the human body. In this study, lightweight, waterproof, mechanically robust, and thermal insulating polyamide-imide (PAI) grooved micro/nanofibrous aerogels were efficiently and directly assembled by electrospinning.
View Article and Find Full Text PDFDetection and characterization of pathogenic Bacillus haynesii from Tribulus terrestris extract: ways to reduce its levels.
Braz J Microbiol
January 2025
Innovation and Drug Discovery, Sava Healthcare Limited, Research Center, MIDC, Block D1, Plot No. 17/6, Chinchwad, Pune, 411019, India.
Plant parts such as roots, bark, leaves, flowers, and fruits that hold ethnopharmacological significance are naturally prone to microbial contamination, influenced by environmental factors like moisture and humidity. This study focuses on assessing the microbial load in the raw material of Tribulus terrestris (TT). The primary bacterium isolated from the pulverized raw material was identified as Bacillus haynesii through 16S rRNA sequencing.
View Article and Find Full Text PDFMg-B-O Coated P2-Type Hexagonal NaMnNiO as a High-Performance Cathode for Sodium-Ion Batteries.
ACS Appl Mater Interfaces
January 2025
National Base for International Science & Technology Cooperation, National Local Joint Engineering Laboratory for Key materials of New Energy Storage Battery, Hunan Province Key Laboratory of Electrochemical Energy Storage and Conversion, School of Chemistry, Xiangtan University, Xiangtan 411105, China.
P2-type NaMnNiO as the cathode for sodium-ion batteries, has a relatively high theoretical specific capacity, but its unstable crystal structure and undesirable phase transitions lead to rapid capacity decay. In this work, Mg-B-O coated NaMnNiO microspheres have been synthesized via a liquid-phase method based on solvothermal synthesized NaMnNiO. The Mg-B-O coating layer significantly improves the electrochemical performance, including specific capacity, rate capability, and cycle stability.
View Article and Find Full Text PDFComprehensive Hematological and molecular Characterization of hemoglobin Hekinan [α27(B8)Glu→Asp(α1), :c.84G > T] in a Large Thai cohort.
Hematology
December 2025
Division of Hematology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.
Background: Hemoglobin (Hb) Hekinan is a prevalent α-globin variant frequently missed in thalassemia screening centers using high-performance liquid chromatography (HPLC) or capillary electrophoresis. This study aims to investigate the hematological and molecular characteristics of Hb Hekinan in a large cohort.
Methods: Hb variants were identified using isoelectric focusing (IEF) and HPLC.
Plasma Circulating Metabolites Associated With Steatotic Liver Disease and Liver Enzymes: A Multiplatform Population-Based Study.
Gastro Hep Adv
September 2024
Department of Epidemiology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Background And Aims: Steatotic liver disease (SLD) is the most common chronic liver disease strongly associated with metabolic dysfunction, but its pathogenesis remains incompletely understood. Exploring plasma circulating metabolites may help in elucidating underlying mechanisms and identifying new biomarkers for SLD.
Methods: We examined cross-sectionally the association between plasma metabolites and SLD as well as liver enzymes using data from 4 population-based cohort studies (Rotterdam study, Avon Longitudinal Study of Parents and Children, The Insulin Resistance Atherosclerosis Family Study, and Study of Latinos).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!