Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Amount of ATP in HeLa cells in various phases of growth was measured after He-Ne laser irradiation (100 J/m2, 10 W/m2, 10 s) by a bioluminescent luciferin-luciferase method. In cells of the exponential phase of growth, the amount of ATP (basal level 8 x 10(-16) mole/cell) starts to increase in 15 min after the irradiation with a maximum (170% above the basal level) at 20 min, and then decreases gradually to the basal level. The sensitivity of the cells to He-Ne laser radiation is lowest in lag-phase of growth, then increases to a plateau (approximately 185% above the control level) from 5th day of cultivation.
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