Chromosomal integration and xylanase gene amplification were demonstrated for the first time in an alkalophilic thermophilic Bacillus sp. (NCIM 59). The integrants were characterized by larger zone of xylan clearance than the parent culture and hybridization with vector (pUC8) DNA. Repeated transformation strategy was used for further amplification of the xylanase gene. The results of Southern blot analysis indicated the occurrence of homologous recombination in the 6.5 kb xylanase gene region of the genomic DNA and suggested a non campbell mode of recombination. The integrants were checked for xylanase production up to ten subcultures and consistently showed two fold higher xylanase activity than the parent strain with the maximum xylanase productivity (U/ml/h) at 16 h.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.1993.2113 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Alleviating -Induced Intestinal Lesions in Chickens Using the Xylanase Xyn10C and Its Binary Cocktail with a Protease.
Animals (Basel)
January 2025
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of were analyzed for their ability to alleviate -induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no challenge; Cp, no enzyme, challenge; Xyn, Xyn10C plus challenge; Xyn+Am, Xyn10C+Amylase plus challenge; Xyn+Ap, Xyn10C+Alkaline protease plus challenge).
View Article and Find Full Text PDFSynergistic action of multiple degumming-related enzymes secreted by Bacillus subtilis XW-18: Decisive factor for driving the bio-degumming process of raw pineapple leaves.
Int J Biol Macromol
January 2025
College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China. Electronic address:
Degumming, a process of removing gummy substances surrounding fiber, plays a crucial role in preparing plant fibers. This study clearly clarified that the multiple degumming enzymes by Bacillus subtilis XW-18 acted as a decisive factor for driving bio-degumming process of raw pineapple leaves. Firstly, PCR analysis verified that B.
View Article and Find Full Text PDFGenomic analysis and synergistic effect with cellulase by Streptomyces thermocarboxydus 12219.
Int J Biol Macromol
January 2025
College of Forestry, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:
In this study, we fully sequenced and analyzed the genome of strain 12219 and identified it as Streptomyces thermocarboxydus. The genome contained a single linear chromosome, 6,950,031 bp in size, with a GC content of 72.21 %.
View Article and Find Full Text PDFEffective Gene Expression Prediction and Optimization from Protein Sequences.
Adv Sci (Weinh)
January 2025
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
High soluble protein expression in heterologous hosts is crucial for various research and applications. Despite considerable research on the impact of codon usage on expression levels, the relationship between protein sequence and expression is often overlooked. In this study, a novel connection between protein expression and sequence is uncovered, leading to the development of SRAB (Strength of Relative Amino Acid Bias) based on AEI (Amino Acid Expression Index).
View Article and Find Full Text PDFTranscription Factors ClrB and XlnR and Their Effect on the Transcription of Cellulase Genes in the Filamentous Fungus .
Int J Mol Sci
December 2024
Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow 119071, Russia.
The filamentous fungus (anamorph ) has been shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms of regulation of cellulase gene expression in the fungus is a current task in industrial biotechnology, since it allows for targeted changes in the composition of the complex secreted by the fungus. Expression of cellulase genes in fungi is regulated mainly at the level of transcription via pathway-specific transcription factors (TF), the majority of which belong to the Zn(II)2Cys6 family of zinc binuclear cluster proteins.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!