Computerized measurement of ciliary beat frequency.

We describe how ciliary beat frequency (CBF) of human respiratory epithelium in vitro can be robustly and quickly measured. The proven photometric technique has been improved by using a microcomputer to calculate and display the results. The software, which runs on an IBM-compatible PC, was developed using Borland Turbo Pascal and employs a fast Fourier transform routine to derive the CBF. Results are displayed almost as soon as a cell is identified under the microscope, and are in accordance with previous work. The technique can now be used diagnostically and for a variety of drug trials on respiratory epithelium.