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Comparison of radial immunodiffusion, turbidimetric immunoassay, and Brix refractometry for determining bovine colostrum quality.
JDS Commun
November 2024
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Determining the concentration of IgG in colostrum is critical for assessment of colostrum quality. On-farm use of a Brix refractometer to estimate colostrum IgG concentration is widespread, whereas radial immunodiffusion (RID) is the laboratory reference method. Turbidimetric immunoassay (TIA) might offer an alternative method to quantify IgG in colostrum, but the agreement with RID, as well as critical thresholds to determine high-quality colostrum, remain uncertain.
View Article and Find Full Text PDFCollaborative study on the cross-reactivity of two influenza B viral components in single radial immunodiffusion assay using quadrivalent influenza vaccines in Japan from 2015/16 to 2021-22 influenza season.
Biologicals
November 2024
Department of Virology III, Gakuen 4-7-1, Musashi-Murayama, Tokyo, 208-0011, Japan; Center for Quality Management Systems, Gakuen 4-7-1, Musashi-Murayama, Tokyo, 208-0011, Japan; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo, 208-0011, Japan. Electronic address:
A quadrivalent influenza vaccine (QIV) has been available in Japan since the 2015/2016 influenza season. Single radial immunodiffusion (SRID) assays are currently used worldwide to measure the hemagglutinin (HA) content of influenza vaccine components because they are simple, accurate, and the regulatory requirement, ensuring consistency in manufacture for the HA content. However, the cross-reactivity of antisera against the two lineages of the influenza B virus (IFVB) may cause inaccurate quantification of HA content in QIVs using the SRID assay.
View Article and Find Full Text PDFA Rapid Virus-Free Method for Producing Influenza HA Immunogen Needed for Preparation of Influenza Vaccine Potency Antisera Reagents.
Influenza Other Respir Viruses
October 2024
Division of Viral Products, Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Background: The potency of inactivated and recombinant influenza vaccines is measured using the single-radial immunodiffusion (SRID) assay. The strain-specific antigen and antibody potency reagents required for the assay are prepared and distributed by regulatory agencies to ensure vaccine standardization, but timely reagent production is always challenging. This poses unique concerns for rapid pandemic responses.
View Article and Find Full Text PDFGenetic and environmental determinants of immunoglobulin G in kid serum and adult colostrum of dairy goats.
J Dairy Sci
January 2025
GenPhySE, Université de Toulouse, INRAE, ENVT, 31326, Castanet Tolosan, France. Electronic address:
Immune competence is a key factor in the health and resilience of dairy ruminants. The aim of this study was to study IgG concentration in serum shortly after birth and in colostrum of lactating animals as proxy of passive immune transfer and colostrum quality, respectively, and thus provide new health and resilience indicators for selection. Data from 424 female kids and 203 primiparous goats, reared on an INRAE experimental farm, were analyzed.
View Article and Find Full Text PDFA human monoclonal antibody based immunoenzymetric assay to measure Fel d 1 concentrations in cat hair and pelt allergenic extracts.
Front Allergy
July 2024
Division of Allergy and Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
In the United States, 19 allergen extracts of different specificities are standardized, which means that their potencies are determined in comparison to a US reference standard. For cat allergen extracts, potency is determined by measuring Fel d 1 content expressed in in Fel d 1 units, and with a unitage that correlates with skin test reactions (bioequivalent allergy units or BAU). Currently, Fel d 1 content is measured with a radial immunodiffusion (RID) assay that uses polyclonal sheep antisera to detect the allergenic protein by producing a white precipitin line in agar gel.
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