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http://dx.doi.org/10.1016/0076-6879(93)21029-8 | DOI Listing |
Chem Mater
March 2024
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
Mixed ionic/electronic conducting polymers are versatile systems for, e.g., energy storage, heat management (exploiting electrochromism), and biosensing, all of which require electrochemical doping, i.
View Article and Find Full Text PDFSmall
October 2023
Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China.
The utilization of dendritic cell (DC) vaccines is a promising approach in cancer immunotherapy, and the modification of DCs for the expression of tumor-associated antigens is critical for successful cancer immunotherapy. A safe and efficient method for delivering DNA/RNA into DCs without inducing maturation is beneficial to achieve successful DC transformation for cell vaccine applications, yet remains challenging. This work presents a nanochannel electro-injection (NEI) system for the safe and efficient delivery of a variety of nucleic acid molecules into DCs.
View Article and Find Full Text PDFMethods Mol Biol
October 2022
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.
The fusion of vesicles and exocytosis release of neurotransmitters into the extracellular space for detection and chemical signal decoding by neighboring cells is the key process in neuronal communication. It is important to understand what regulates exocytosis because the amount of neurotransmitters released into the synaptic cleft has a direct impact on brain function such as cognition learning and memory as well as on brain malfunctions. Much success in molecular biology can be credited for the existence of simplified model systems.
View Article and Find Full Text PDFAdv Ther (Weinh)
December 2019
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
Transfection is a critical step for gene editing and cell-based therapies. Nanoscale technologies have shown great promise to provide higher transfection efficiency and lower cell perturbation than conventional viral, biochemical and electroporation techniques due to their small size and localized effect. Although this has significant implications for using cells post-transfection, it has not been thoroughly studied.
View Article and Find Full Text PDFMethods Mol Biol
January 2021
School of Biochemistry, University of Bristol, Bristol, UK.
The ability to microinject substances into the cytosol of living neutrophils opens the possibility of manipulating the chemistry within the cell and also of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method. Here we outline two techniques which work well with neutrophils, namely, SLAM (Simple Lipid-Assisted Microinjection) and electromicroinjection.
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