In order to calculate the atherogenic coefficient the authors have made a comparative estimation of methods for determining alfa-cholesterin in serum applying various temperature regimes during sedimentation of beta and pre-beta-lipoproteids. It was found out that the complete sedimentation of beta and pre-beta-lipoproteids could be obtained after incubation of specimen into the ice bath for 30 min. (t 0-4 degrees C) with further centrifugation at room temperature. No differences were disclosed in comparison with the reference method based on refrigerated centrifugation at 2-4 degrees C.
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