A total of 176 reference strains including 63 species of Enterobacteriaceae mainly of clinical origin were tested by commercial diagnostic kits ENTEROtest 1 & 2. The identification efficacy in both systems TNW and IDENTI was nearly in agreement 144 (81.8%), 142 (80.7%) respectively correctly identified strains. The NIS 86 system identified only 54 (30.7%) strains correctly.
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Protocol for cell image-based spatiotemporal proteomics in budding yeast.
STAR Protoc
January 2025
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:
The eukaryotic cell division cycle is a highly conserved process, featuring fluctuations in protein localization and abundance required for key cell cycle transitions. Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. We describe steps for strain construction, cell cultivation, microscopy, and image analysis.
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BMC Microbiol
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School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
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Infect Agent Cancer
January 2025
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