Using PCR and an experimentally validated V alpha subfamily-specific oligonucleotide panel (V alpha 1-w29), we have investigated whether the TCR delta chain may increase its combinatorial diversity by using V genes considered as alpha chain-specific. We show that at least 10 distinct human V alpha segments rearrange at the J delta locus, leading to scrambling of the two V gene repertoires. Fifty-five per cent of the V alpha/J delta transcripts characterized here were in frame. The 17 V alpha/C delta chains analysed included an extended CDR3 region with up to 18 aa encoded by the junctional region. In addition, a new J delta segment (J delta 4) has been characterized. Together, these findings demonstrate that combinatorial diversity in the human delta locus is larger than previously thought.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0161-5890(94)90083-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular and cellular targets of GABAergic anesthetics in the mesopontine tegmentum that enable pain-free surgery.
Pain
December 2024
Department of Cell and Developmental Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
The mesopontine tegmental anesthesia area (MPTA) is a focal brainstem locus which, when exposed to GABAergic agents, induces brain-state transitioning from wakefulness to unconsciousness. Correspondingly, MPTA lesions render animals relatively insensitive to GABAergic anesthetics delivered systemically. Using chemogenetics, we recently identified a neuronal subpopulation within the MPTA whose excitation induces this same pro-anesthetic effect.
View Article and Find Full Text PDFThe tomato gene Ty-6, encoding DNA polymerase delta subunit 1, confers broad resistance to Geminiviruses.
Theor Appl Genet
January 2025
Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands.
The tomato Ty-6 gene conferring resistance against begomoviruses has been cloned and shown to be a variant of DNA polymerase delta subunit 1. Ty-6 is a major resistance gene of tomato that provides resistance against monopartite and bipartite begomoviruses. The locus was previously mapped on chromosome 10, and in this study, we fine-mapped Ty-6 to a region of 47 kb, including four annotated candidate genes.
View Article and Find Full Text PDFPersonality and Situational Determinants of Sustained Helping Towards Ukrainian War Refugees in 2022.
Psychol Res Behav Manag
December 2024
Institute of Psychology, Faculty of Social Sciences, University of Opole, Opole, Poland.
Article Synopsis
- The research aimed to identify personality and situational predictors that influence people's willingness to help Ukrainian war refugees in 2022.
- A survey was conducted with 720 participants in March and 127 in June 2022, measuring factors like personality traits, altruism, anxiety, and social distance towards Ukrainians and Russians.
- Findings showed that higher altruism and certain situational factors, such as anxiety and attitudes towards Russia, significantly contributed to ongoing support for refugees, with altruistic individuals helping regardless of their perceived threat level.
A Genetic Variant of Delta-9 Desaturase Is Associated With Latitudinal Adaptation in a Coral from the Great Barrier Reef.
Mol Ecol
December 2024
Department of Integrative Biology, University of Texas at Austin, Austin, Texas, USA.
Coral populations across the Great Barrier Reef (GBR) could rapidly adapt to the warming climate if they have standing genetic variation for thermal tolerance. Here, we describe a locus likely involved in latitudinal adaptation of Acropora millepora. This locus shows a steep latitudinal gradient of derived allele frequency increasing at higher latitudes, and harbours a cluster of eight tandemly repeated Δ9-desaturase genes adjacent to a region in the genome where a hard selective sweep likely occurred.
View Article and Find Full Text PDFGenes of DLK1-DIO3 Locus and miR-379/656 Cluster is a Potential Diagnostic and Prognostic Marker in Patients With Hepatocellular Carcinoma: A Systems Biology Study.
J Clin Exp Hepatol
November 2024
Center of Excellence in Molecular Biology and Regenerative Medicine, Department of Biochemistry, JSS Medical College, Mysuru, India.
Background: Hepatocellular carcinoma is the sixth most common malignancy reported globally. This highlights the need for reliable biomarkers that can be employed for diagnostic and prognostic applications. The present study aimed to classify and characterize the clinical potential of delta like non-canonical Notch ligand 1-type III iodothyronine deiodinase (DLK1-DIO3) and miR-379/656 cluster genes in hepatocellular carcinoma.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!