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A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees.
Sci Rep
January 2025
Institute for Biosecurity and Microbial Forensics (IBMF), Oklahoma State University, Stillwater, OK, USA.
Exclusion is a keystone of integrated pest management to prevent the introduction of pathogens. U.S.
View Article and Find Full Text PDFVariation of gene ratios in mock communities constructed with purified 16S rRNA during processing.
Sci Rep
December 2024
Department of Chemical Engineering, Polytechnic School, University of São Paulo, Av. Prof. Luciano Gualberto, Travessa 3, n. 380., São Paulo, SP, CEP 05508-900, Brazil.
16S ribosomal nucleic acid (16S rRNA) analysis allows to specifically target the metabolically active members of microbial communities. The stability of the ratios between target genes in the workflow, which is essential for the bioprocess-relevance of the data derived from this analysis, was investigated using synthetic mock communities constructed by mixing purified 16S rRNA from Bacillus subtilis (Bs), Staphylococcus aureus (Sa), Pseudomonas aeruginosa (Pa), Klebsiella pneumoniae (Kp) and Burkholderia cepacia (Bc) in different proportions. The RT reaction yielded one copy of cDNA per rRNA molecule for Pa, Bc and Sa but only 2/3 of the expected cDNA from 16S rRNAs of Bs and Kp.
View Article and Find Full Text PDFStructure of Genes Encoding Oxidosqualene Cyclases-Key Enzymes of Triterpenoid Biosynthesis from Sea Cucumber .
Int J Mol Sci
November 2024
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Pr. 100 let Vladivostoku, 690022 Vladivostok, Russia.
Article Synopsis
- OSCs (Oxidosqualene cyclases) are important enzymes that change linear triterpenes into tetracyclic compounds, which then serve as precursors for bioactive metabolites.
- Two OSC genes associated with different synthases, parkeol and lanostadienol, were identified in sea cucumbers from the Sclerodactylidae family to expand knowledge on OSC diversity.
- Analysis revealed that the two identified OSC genes, OSC1 and OSC2, share a high degree of sequence similarity and suggest distinct evolutionary paths from OSC genes found in the Stichopodidae family.
Identification and pharmacological characterization of pH-sensitive chloride channels in the fall armyworm, Spodoptera frugiperda.
Insect Biochem Mol Biol
December 2024
The Key Laboratory of Agri-products Quality and Biosafety, Ministry of Education, Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, PR China. Electronic address:
Article Synopsis
- pH-sensitive chloride channels (pHCls) are found only in invertebrates and are vital for processes like fluid regulation and food intake, with this study focusing on two specific subunits, SfpHCl1 and SfpHCl2, from the fall armyworm.
- Both subunits function similarly when expressed in frog oocytes, showing chloride selectivity with their activity impacted by changes in extracellular pH and activated by zinc ions and the insecticide emamectin benzoate.
- The study reveals significant differences in activation and deactivation properties between SfpHCl1 and SfpHCl2, highlighting their potential as targets for developing specialized insecticides.
Comparative Characterization of Three Homologous Glutathione Transferases from the Weed .
Foods
November 2024
Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855 Athens, Greece.
The comparative analysis of homologous enzymes is a valuable approach for elucidating enzymes' structure-function relationships. Glutathione transferases (GSTs, EC. 2.
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