AI Article Synopsis
- The study analyzed the effectiveness of two promoters in Brevibacterium sp. R312 using the cat reporter gene, with an emphasis on how well they performed compared to their expression in E. coli.
- The tac promoter showed poor expression in Brevibacterium sp., indicating that it may not be suitable for use in this bacteria.
- In contrast, the AatII-SalI fragment from plasmid pYEJ001 demonstrated strong activity, producing the chloramphenicol acetyltransferase (CAT) enzyme, confirmed by a detectable 25,600-kDa protein in the cell extracts.
Article Abstract
The cat reporter gene was used to assess expression of two promoters, previously strongly expressed in Escherichia coli, in Brevibacterium sp. R312 strain. The tac promoter (de Boer et al., 1983, Proc. Natl. Acad. Sci. USA 80, 21-25) was poorly expressed in Brevibacterium sp. In contrast, the AatII-SalI fragment of plasmid pYEJ001 (Pharmacia LKB Biotechnology, Sweden) containing two lac operators, a consensus sequence promoter and the cat structural gene clearly revealed chloramphenicol acetyltransferase activity and the presence of a 25,600-kDa protein, corresponding to the monomeric CAT protein, in cell extracts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/plas.1993.1061 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!