AI Article Synopsis
- A new cytolysin gene called slyA has been found in Salmonella, which causes hemolysis on blood agar and appears to be distinct from other toxins.
- The slyA gene is consistently present in all Salmonella strains tested, as well as in related bacteria like Shigella and certain E. coli, but is absent in other Enterobacteriaceae.
- Purified slyA, known as salmolysin, exhibits hemolytic and cytolytic properties, and studies indicate it plays a crucial role in Salmonella's virulence by helping it survive within mouse macrophages.
Article Abstract
A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC42974 | PMC |
| http://dx.doi.org/10.1073/pnas.91.2.489 | DOI Listing |
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